西瓜PIP5K基因家族鉴定及其在雄性不育花蕾中的表达分析  

Identification of PIP5K Gene Family in Watermelon(Citrullus lanatus)and Its Expression Analysis in Male Sterile Floral Buds

作　　者：张高原[1] 丁谦 魏兵强[1] ZHANG Gaoyuan;DING Qian;WEI Bingqiang(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China;College of Floriculture,Weifang Engineering Vocational College,Qingzhou Shandong 262500,China)

机构地区：[1]甘肃农业大学园艺学院,兰州730070 [2]潍坊工程职业学院花卉学院,山东青州262500

出　　处：《西北农业学报》2021年第6期883-893,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：甘肃农业大学公开招聘博士科研启动基金(2017RCZX-30)。

摘　　要：为了挖掘西瓜磷脂酰肌醇4-磷酸5-激酶(PIP5K)基因的功能,利用生物信息学方法对西瓜PIP5K家族成员进行鉴定和特征分析,并对其在多个组织的表达模式进行分析研究。结果显示:西瓜基因组中含有8个PIP5K家族成员,分布在6条染色体上;多数基因定位在细胞质或细胞膜上;共线性分析发现4个ClaPIP5K基因与5个AtPIP5K基因存在共线关系,并且ClaPIP5K基因间存在片段复制事件;进化树分析发现ClaPIP5K基因和AtPIP5K基因被分成4组,并且同一组内的基因具有相似的保守基序和基因结构;启动子分析发现ClaPIP5K基因中含有激素和胁迫响应相关顺式作用元件;表达分析显示ClaPIP5K基因在西瓜生长点、果柄、果肉、叶片、下胚轴、根以及花蕾等组织中表现出不同的表达水平。In order to explore the function of phosphatidylinositol 4-phosphate 5-kinase(PIP5K)genes in watermelon,the watermelon PIP5K gene family was identified and their characteristics and expression patterns were analyzed by using bioinformatics method in this study.The results showed that there were 8 PIP5K members in watermelon,which were distributed on six chromosomes.Most genes were located in cytoplasm or cell membrane.Syntenic analysis showed that there were synteny relationships between 4 ClaPIP5K and 5 AtPIP5K genes,and one ClaPIP5K gene pair was identified as segmental duplication.The phylogenetic tree analysis showed that ClaPIP5K and AtPIP5K genes were divided into 4 groups,and the genes had similar conserved motifs and gene structure in the same group.The promoter analysis showed that the ClaPIP5K genes contained cis-acting elements related to hormone and stress response.The expression pattern analysis showed that ClaPIP5K genes displayed different expression levels in the apical point,carpopodium,fruit flesh,leaf,hypocotyl,root and floral bud of watermelon.

关 键 词：西瓜 PIP5K 基因家族 雄性不育 表达模式 

分 类 号：S651[农业科学—果树学]