硬枝碱蓬多糖通过肠肌间神经丛抑制家兔离体十二指肠收缩及机制  

The mechanism of Suaeda rigida polysaccharides inhibiting the isolated duodenal contraction in rabbits through the myenteric plexus

作　　者：沙爱龙 郝海燕 SHA Ailong;HAO Haiyan(School of Teacher Education,Chongqing Three Gorges University,Chongqing 404120,China;School of Biology and Food Engineering/Chongqing Research Center for the Development and Utilization of Northeast Chongqing Characteristic Biological Resources,Chongqing Three Gorges University,Chongqing 404120,China;School of Environmental and Chemical Engineering/Chongqing Key Laboratory of Water Environment Evolution and Pollution Control in Three Gorges Reservoir Area,Chongqing Three Gorges University,Chongqing 404120,China)

机构地区：[1]重庆三峡学院教师教育学院,重庆404120 [2]重庆三峡学院生物与食品工程学院/重庆市渝东北特色生物资源开发利用工程技术研究中心,重庆404120 [3]重庆三峡学院环境与化学工程学院/三峡库区水环境演变与污染防治重庆市重点实验室,重庆404120

出　　处：《中山大学学报（自然科学版）（中英文）》2021年第4期19-25,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金(31360635);重庆市教育委员会科学技术研究项目(KJQN201901235,KJQN202001226);重庆市渝东北特色生物资源开发利用工程技术研究中心开放基金项目(19sgxyjdzz12);重庆三峡学院人才引进科研启动金项目(20190002)。

摘　　要：研究了不同剂量的硬枝碱蓬多糖对家兔离体十二指肠收缩活动的影响,并探究其作用机制。利用Medlab生物信号采集处理系统记录用药前后各组家兔离体十二指肠的收缩活动,观察硬枝碱蓬多糖低、中、高三个剂量组对肠收缩频率和幅度的影响。分别选取Ach、CaCl_(2)与高剂量硬枝碱蓬多糖共同孵育,观察对十二指肠收缩的影响。用ELISA法检测不同剂量的硬枝碱蓬多糖对家兔离体十二指肠中亮氨酸脑啡肽(Leu-enk)、甲硫氨酸脑啡肽(Met-enk)含量和酪氨酸羟化酶(TH)活性的影响。结果显示,与给药前相比,硬枝碱蓬多糖3个剂量组对家兔离体十二指肠收缩频率和幅度均具有抑制作用,其中低剂量组抑制不显著(P>0.05)、中剂量组抑制显著(P<0.05)、高剂量组抑制极显著(P<0.01),均呈现一定的剂量依赖效应。高剂量硬枝碱蓬多糖均能极显著(P<0.01)抑制Ach、CaCl_(2)分别对肠收缩频率和幅度的促进作用,抑制效果分别与硫酸阿托品和盐酸维拉帕米相当。ELISA实验表明,与正常对照组相比,硬枝碱蓬多糖3个剂量组家兔离体十二指肠中Leu-enk和Met-enk含量均不同程度减少,TH活性均不同程度升高。上述结果提示,硬枝碱蓬多糖能抑制家兔离体十二指肠收缩,作用机制可能是通过肠肌间神经丛抑制G蛋白耦联M受体介导的AC-cAMP-PKA和PLC-IP3-Ca^(2+)信号转导通路及Ca^(2+)信号系统(如抑制肌膜ICa-L进而抑制Ca^(2+)-CaM信号通路);可能通过抑制肌间神经丛运动神经元释放Leu-enk和Met-enk,从而抑制G蛋白耦联δ受体介导的GTP-cAMP-(PKK或PKC)信号通路及Ca^(2+)信号系统;并可能通过促进肌间神经丛运动神经元释放TH引起NE增多,再激活G蛋白耦联β受体介导的AC-cAMP-PKA信号转导通路。The study aims to investigate the effect of different dosages of Suaeda rigida polysaccharides on the contractile activity of the isolated rabbit duodenum and explore its mechanism.The contractile activity of the isolated duodenum was recorded by Medlab biological signal acquisition and processing system before and after administration,and the effects of Suaeda rigida polysaccharides-treated groups of the low,medium,and high doses on the contractile frequency and amplitude of the intestines were observed.Acetylcholine(ACh)and CaCl_(2) were selected respectively to be co-incubated with the high dose of Suaeda rigida polysaccharides to observe the effects on the duodenal contraction.The effects of different doses of Suaeda rigida polysaccharides on the contents of Leu enkephalin(Leu-enk),met enkephalin(Met-enk)and the activity of tyrosine hydroxylase(TH)in the isolated rabbit duodenum were detected by enzyme-linked immunosorbent assay(ELISA).The results showed that the three dose groups of Suaeda rigida polysaccharides had inhibitory effects on the contractile frequency and amplitude of the isolated duodenum in rabbits compared with those before administration.Among them,the inhibition of the low dose group was not significant(P>0.05),the medium dose group was significant(P<0.05),and the high dose group was very significant(P<0.01),showing a dose-dependent effect.The high dose of Suaeda rigida polysaccharides could significantly(P<0.01)inhibit the promotion effects of ACh and CaCl_(2) on the frequency and amplitude of intestinal contraction,and the inhibitory effects were similar to those of atropine sulfate and verapamil hydrochloride,respectively.The results of ELISA showed that compared with the control group,the contents of Leu-enk and Met-enk in the isolated rabbits duodenum in the three groups of Suaeda rigida polysaccharides were decreased in varying degrees,and the activity of TH was increased in varying degrees.These results indicated that Suaeda rigida polysaccharides can inhibit the contraction of the is

关 键 词：硬枝碱蓬多糖 十二指肠平滑肌 肌间神经丛 神经递质 

分 类 号：R965[医药卫生—药理学] R285.5[医药卫生—药学]