A 36 Mb terminal deletion of chromosome 2BL is responsible for a wheat semi-dwarf mutation  

作　　者：Qiuhong Wu Yongxing Chen Jingzhong Xie Lingli Dong Zhenzhong Wang Ping Lu Rongge Wang Chengguo Yuan Yan Zhang Zhiyong Liu 

机构地区：[1]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]China Agricultural University,Beijing 100193,China [4]Hebei Gaoyi Stock Seed Farm,Gaoyi 051330,Hebei,China

出　　处：《The Crop Journal》2021年第4期873-881,共9页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFD0100302);the Science and Technology Service Network Initiative of Chinese Academy of Sciences(KFJ-STS-ZDTP-024)。

摘　　要：Reduced plant height is one of the most important traits related to lodging resistance and crop yield. The use of reduced height genes has been one of the main features in breeding modern high-yielding wheat varieties with less lodging. A spontaneous dwarf mutant DD399 was identified in a high yielding, gibberellic acid(GA)-insensitive, lodging-resistant variety Nongda 399(ND399). Significant differences in upper internode lengths between mutant DD399 and wild type ND399 were caused by reduced cell elongation. The plant height of ND399 × DD399 F_(1) hybrids was intermediate between the parents, indicating incomplete dominance or a dose–response effect of a reduced height gene. Plant height showed continuous distribution in the F_(2) population, and segregation distortion was observed among the 2292 F_(2:3) progenies. The reduced height mutation was characterized by Illumina 90 K iSelect SNP genotyping and bulked segregant RNA-Seq(BSR-Seq) analysis of the segregating population. A concentrated cluster of polymorphic SNPs associated with the reduced height phenotype was detected in the distal region of chromosome arm 2 BL. Co-segregation of reduced height phenotype with the clustered markers revealed a 36 Mb terminal deletion of chromosome 2 BL in mutant DD399.

关 键 词：BSR-Seq Reduced height Semi-dwarf stature SNP Triticum aestivum 

分 类 号：S512.1[农业科学—作物学]