Genome-wide characterization of early response genes to abscisic acid coordinating multiple pathways in Aegilops tauschii  

作　　者：Yu Wei Liangjing Cao Xucheng Huang Xuan Wang Huan Wang Yongchao Song Qiang He Mingjie Lyu Xinwen Hu Jun Liu 

机构地区：[1]National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,Hainan,China [3]Department of Biological Sciences,Xi’an Jiaotong-Liverpool University,Suzhou 215123,Jiangsu,China [4]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2021年第4期934-944,共11页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFD0101001);the Agricultural Science and Technology Innovation Program of CAAS。

摘　　要：The diploid wild goat grass Aegilops tauschii(Ae. tauschii, 2 n = 14;DD), as the D-sub genome of common wheat, provides rich germplasm resources for many aspects of wheat breeding. Abscisic acid(ABA) is an essential phytohormone that plays a pivotal role in plant adaptation to abiotic stresses. However,the gene regulation network of Ae. tauschii in response to ABA stress remains unclear. Here, we conducted a time-course strand-specific RNA-sequencing study to globally profile the transcriptome that responded to ABA treatment in Ae. tauschii. We identified 4818 differentially expressed transcription units/genes with time-point-specific induction/repression patterns. Using functional annotation, one-to-one ortholog and comparative transcriptome profiling analyses, we identified 319 ABA-responsive Ae. tauschii orthologs that were also induced/repressed under ABA treatment in hexaploid wheat. On the quantitative trait loci(QTL) used in wheat marker-assisted breeding, we found that the ABA-responsive expression patterns of eight Ae. tauschii orthologs were associated with drought stress tolerance, flowering process and/or grain quality. Of them, the ABA-responsive gene encoding sucrose:sucrose 1-fructosyltransferase in fructan and glucose metabolism pathways showed the most significant association with wheat drought tolerance. The characterization of ABA early-responsive genes in this study provides valuable information for exploring the molecular functions of the regulatory genes and will assist in wheat breeding.

关 键 词：WHEAT Aegilops tauschii TRANSCRIPTOME Abscisic acid FRUCTAN 

分 类 号：S512.1[农业科学—作物学]