德宏州油茶炭疽病拮抗内生芽孢杆菌6715的筛选与鉴定  

Screening and Identification of Bacillus tequilensis 6715 as Biological Agent Against Camellia oleifera Anthracnose Disease in Dehong Prefecture

作　　者：周嫒婷 王芳 尹加笔 刘丽[1] 张东华[1] 洪英娣[1] 沈德周 马焕成 伍建榕[1,2] ZHOU Ai-ting;WANG Fang;YIN Jia-bi;LIU Li;ZHANG Dong-hua;HONG Ying-di;SHEN De-zhou;MA Huan-cheng;WU Jian-rong(College of Biodiversity Conservation,Southwest Forestry University,Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions,Kunming Yunnan 650224,P.R.China;College of Forestry,Southwest Forestry University,Key Laboratory for Biodiversity Conservation in Southwest China of State Forestry and Grassland Administration,Kunming Yunnan 650224,P.R.China;Dehong State Forestry and Grassland Bureau,Dehong Yunnan 678400,P.R.China)

机构地区：[1]西南林业大学生物多样性保护学院,云南省高校森林灾害预警控制重点实验室,云南昆明650224 [2]西南林业大学林学院,国家林业和草原局西南地区生物多样性保育重点实验室,云南昆明650224 [3]德宏州林业和草原局,云南德宏678400

出　　处：《西部林业科学》2021年第4期131-138,共8页Journal of West China Forestry Science

基　　金：国家自然科学基金项目(31860208、31560207);国家重点研发计划(2019YFD100200X);西南林业大学木棉纤维人工林产业化培育省级创新团队项目(2018HC014)。

摘　　要：为减轻德宏州油茶炭疽病的危害和保证油茶产品质量,特采集该地区健康叶片,用组织分离法分离内生细菌,并通过平板对峙培养法测定所分离内生菌对油茶炭疽菌的抑制活性,后回接离体叶片对具有显著抑菌效果的内生菌验证其防效,最后用形态学及16S rRNA序列分析对抑菌效果最好的菌株进行鉴定,并通过PCR扩增脂肽基因。结果显示,从健康油茶叶片中共分离到25株内生细菌,其中菌株6715对油茶炭疽菌平板抑制效果最好,抑制率为57.59%,回接叶片发现其本身不致病,并显著抑制炭疽菌发生,经形态学和16S rRNA序列分析鉴定为Bacillus tequilensis,且扩增出脂肽基因bacA、ppsD和srfAA,具有潜在的生防价值。In order to reduce the anthracnose disease damage of Camellia oleifera and ensure the quality of C.oleifera products in Dehong Prefecture in Yunnan Province,healthy leaves of C.oleifera collected,and endophytic bacteria were isolated by tissue separation method,then the antagonistic activity of isolated endophytic bacterial strains against Colletotrichum fructicola was performed using the dual-culture plate approach,subsequently,morphological and 16 S rRNA sequence analysis were used to identify the strains with the best bacteriostatic effect,and its lipopetide antibiotic biosynthesis genes were analyzed.The results showed that 25 bacteria strains were isolated from healthy leaves of C.oleifera.Successive screenings of these 25 isolates yielded strain 6715 as the best antagonist against Colletotrichum fructicola with an efficacy of 57.59%in vitro and significantly reduced the anthracnose disease in C.oleifera detached leaves.Then the strain 6715 was identified as Bacillus tequilensis.Furthermore,PCR detection showed that the strain 6715 had the lipopetide antibiotic biosynthesis genes:bacA,ppsD and srfAA.Using the B.tequilensis strain 6715 may have the potential biocontrol value for prevention C.oleifera anthracnose disease.

关 键 词：油茶 内生细菌 炭疽菌 鉴定 拮抗作用 

分 类 号：S763.7[农业科学—森林保护学]