Rapid Magnetic 3D Printing of Cellular Structures with MCF-7 Cell Inks  

作　　者：S.Mishriki A.R.Abdel Fattah T.Kammann R.P.Sahu F.Geng I.K.Puri 

机构地区：[1]School of Biomedical Engineering,McMaster University,Hamilton,Ontario,Canada [2]Department of Mechanical Engineering,McMaster University,Hamilton,Ontario,Canada [3]Faculty of Biological Sciences,Friedrich-Schiller-University Jena,Germany

出　　处：《Research》2019年第1期1183-1195,共13页研究（英文）

基　　金：The authors thank Kerollos Barsoum for supplying reagents for PCR analysis.This work was supported by the Natural Sciences and Engineering Research Council of Canada(NSERC)Discovery Grant[RGPIN-2014-04066];the NSERC Engage Grant[EGP 513882-17];Canada Foundation for Innovation John R.Evans Leaders Fund(CFI-JELF);the Ontario Research Fund Research Infrastructure[ORF-RI:Grant no.33016];the Mitacs Globalink Program and the German Academic Exchange Service(DAAD).

摘　　要：A contactless label-free method using a diamagnetophoretic ink to rapidly print three-dimensional(3D)scafold-free multicellular structures is described.Te inks consist of MCF-7 cells that are suspended in a culture medium to which a paramagnetic salt,diethylenetriaminepentaacetic acid gadolinium(III)dihydrogen salt hydrate(Gd-DTPA),is added.When a magnetic feld is applied,the host fuid containing the paramagnetic salt is attracted towards regions of high magnetic feld gradient,displacing the ink towards regions with a low gradient.Using this method,3D structures are printed on ultra-low attachment(ULA)surfaces.On a tissue culture treated(TCT)surface,a 3D printed spheroid coexists with a two-dimensional(2D)cell monolayer,where the composite is termed as a 2.5D structure.Te 3D structures can be magnetically printed within 6 hours in a medium containing 25 mM Gd-DTPA.Te infuence of the paramagnetic salt on MCF-7 cell viability,cell morphology,and ability of cells to adhere to each other to stabilize the printed structures on both ULA and TCT surfaces is investigated.Gene expressions of hypoxia-inducible factor 1-alpha(HIF1�)and vascular endothelial growth factor(VEGF)allow comparison of the relative stresses for the printed 3D and 2.5D cell geometries with those for 3D spheroids formed without magnetic assistance.Tis magnetic printing method can be potentially scaled to a higher throughput to rapidly print cells into 3D heterogeneous cell structures with variable geometries with repeatable dimensions for applications such as tissue engineering and tumour formation for drug discovery.

关 键 词：STRUCTURE CULTURE DIMENSIONS 

分 类 号：R73[医药卫生—肿瘤]