磁性纳米Fe_(3)O_(4)@C对SBR脱氮除磷性能及其微生物种群组成的影响  

Effects of Fe_(3)O_(4)@C nanoparticles on nitrogen and phosphorus removal performance and microbial community in a sequencing batch reactor

作　　者：刘琴[1] 信欣[1] 周希 廖娟 段文瑶 解宗兰 叶恒 LIU Qin;XIN Xin;ZHOU Xi;LIAO Juan;DUAN Wenyao;XIE Zonglan;YE Heng(College of Resources and Environment,Chengdu University of Information Technology,Chengdu 610225)

机构地区：[1]成都信息工程大学资源环境学院,成都610225

出　　处：《环境科学学报》2021年第7期2664-2672,共9页Acta Scientiae Circumstantiae

基　　金：四川省教育厅科研项目(No.17ZA0067);大学生创新创业训练计划项目(No.202010621355)。

摘　　要：为探究磁性纳米Fe_(3)O_(4)@C对序批式活性污泥反应器(SBR)污水处理系统脱氮除磷性能的影响,建立了2个相同的SBR(编号分别为0号和1号),其中,0号反应器未投加任何磁性材料(对照组),1号反应器中投加0.5 g·L^(-1)的Fe_(3)O_(4)@C(实验组),并采用高通量测序和实时定量PCR(qPCR)技术对2个反应器内生物种群结构及关键脱氮除磷功能菌群进行分析.结果表明:(1)Fe_(3)O_(4)@C对SBR除污性能有显著影响,其中,1号反应器化学需氧量(COD)的去除性能得到增强,24 d后去除率稳定在90%左右;0号和1号反应器总氮(TN)平均去除率分别为35.83%和52.18%;从第43 d起,1号反应器除磷性能明显高于0号反应器,运行70 d后,0号和1号反应器对总磷(TP)的平均去除率分别为47.52%和56.33%.(2)添加Fe_(3)O_(4)@C材料后,反应器内变形菌门(Proteobacteria)的相对丰度从23.91%增加至32.14%,优势脱氮菌门Planctomycetes、Nitrospirae的相对丰度分别从2.66%、0.46%增加至5.16%、2.23%.(3)添加Fe_(3)O_(4)@C后,SBR内细菌总数明显增多,16S基因拷贝数从1.77×10^(7) copies·g^(-1)增长到4.21×10^(7) copies·g^(-1);各功能菌数量也有大幅度增长,除磷功能基因PAO增加了3.4倍,脱氮功能基因nirS、Nitrospira、Nitrobacter、AOB分别增长了2.3、2.4、4.7和572倍.研究表明,磁性纳米Fe_(3)O_(4)@C能有效促进SBR脱氮除磷性能,可为SBR工艺的优化改进提供理论支撑.The identical two sequencing batch reactors(SBRs)(named by No.0 and No.1)were set up in order to study the effects of magnetic nano-Fe_(3)O_(4)@C on the performances of biological nitrogen and phosphorus removal in a sequencing batch reactor(SBR).The dosage of 0.5 g·L^(-1) nano-Fe_(3)O_(4)@C were added to reactor-1,and no addition of magnetic material in reactor-0.High throughput sequencing and real-time quantitative PCR(qPCR)were used to analyze the key microbial populations in the two reactors.The results showed that:(1)Fe_(3)O_(4)@C had significant influence on the performance of SBR.Among them,COD removal in reactor-1 was enhanced,and the efficiency was stable at about 90%after 24 days;the average efficiency of TN in reactor-0 and-1 were 35.83%and 52.18%,respectively.From day 43,the phosphorus removal performance of reactor 1 was significantly higher than reactor 0.From day 70,the average efficiency of TP in reactor-0 and-1 were 47.52%and 56.33%,respectively.(2)After adding Fe_(3)O_(4)@C,the relative abundance of Proteobacteria increased from 23.91%to 32.14%;the dominant nitrogen removal bacteria Planctomycetes and Nitrospirae increased from 2.66%,to 5.16%and from 0.46%to 2.23%,respectively.(3)After adding Fe_(3)O_(4)@C,the total number of bacteria in SBR increased significantly,and the number of 16 s gene copies increased from 1.77×10^(7) to 4.21×10^(7) copies·g^(-1).the number of functional bacteria also increased significantly,and the phosphorus removal gene PAO increased 3.4-fold;the genes of nirS,Nitrospira,Nitrobacter,and AOB increased by 2.3,2.4,4.7,and 572 times,respectively.Experimental results showed that magnetic nano-Fe_(3)O_(4)@C can effectively promote the nitrogen and phosphorus removal performance in SBRs,providing theoretical support for the optimization and improvement of SBR process.

关 键 词：磁性纳米Fe_(3)O_(4)@C SBR工艺 脱氮除磷 定量PCR 微生物种群 

分 类 号：X703.1[环境科学与工程—环境工程]