溴硝醇有关物质的测定  

Determination of bronopol-related substances

作　　者：向阳 王天威 钟金清 刘雪婷 胡鲲[1,2,3] XIANG Yang;WANG Tianwei;ZHONG Jinqing;LIU Xueting;HU Kun(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources of the Ministry of Agriculture and Rural Affirs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产科学国家级实验教学示范中心,上海201306 [2]上海海洋大学国家水生动物病原库,上海201306 [3]上海海洋大学农业农村部淡水水产种质资源重点实验室,上海201306

出　　处：《生物加工过程》2021年第4期457-462,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2019YFD0900102);上海市科学技术委员会项目(18391901500)。

摘　　要：建立了测定溴硝醇有关物质的高效液相色谱方法,色谱柱为RP ODS(150 mm×4.6 mm,5μm)柱,其流动相为V(磷酸水)∶V(甲醇)=95∶5(磷酸水中,磷酸与水的体积比1∶1000),流速为1.0 mL/min,柱温为30℃,检测波长为210 nm,对溴硝醇开展了专属性、检测限、线性、精密度、再现性、稳定性和耐用性研究。结果表明:溴硝醇与其杂质良好分离,溴硝醇在质量浓度为2-40μg/mL区间内线性关系良好(相关系数R^(2)=0.9999),其平均回收率为102.46%(RSD=1.79%)。在本测定条件下溴硝醇的检测限为0.1μg/mL;定量限为1μg/mL。该方法具有操作简便、准确性高及专属性良好的特点,可用于溴硝醇有关物质的测定。We established a high-performance liquid chromatography(HPLC)method to determine bronopol-related substances.The determination was performed on Agilent HPLC with RP-ODS(150 mm×4.6 mm,5μm)with the mobile phase of phosphoric acid-methyl alcohol(V∶V=95∶5)at a flow rate of 1.0 mL/min,a column temperature of 30℃,and a detection wavelength of 210 nm.In addition the phosphoric acid was composed of phosphoric acid and water at a ratio of 1∶1000(V∶V).The specificity,detection limit,linearity,precision,reproducibility,stability,and durability studies have been carried out for bronopol.The results show that bronopol was well separated from its impurity.Bronopol showed a great linear relationship at concentrations ranging from 2 to 40μg/mL(R^(2) was 0.9999).And the average recovery was 102.46%(RSD=1.79%).The detection limit of bronopol was 0.1μg/mL under this condition.Moreover the limit of quantitation was 1μg/mL.Our finding shows that the method is convenient,accurate and specific to determine bronopol-related substances.

关 键 词：溴硝醇 2硝基1 3丙二醇 HPLC 药物残留 水产养殖 

分 类 号：O657.72[理学—分析化学] TQ450.7[理学—化学]