MicroRNA-188对前列腺癌细胞增殖/凋亡的调控作用以及自拟固本散结方对其干预机制研究  

The regulatory effect of Microrna-188 on the proliferation and apoptosis of prostate cancer cells and the intervention mechanism of Guben Sanjie Formula

作　　者：赖伟业[1] 钟喨[1] 梁桂芳[1] 吴松[1] 刘毅豪[1] LAI Weiye;ZHONG Hang;LIANG Guifang;WU Song;LIU Yihao(Department of Urology,Zhongshan Hospital of TCM,Zhongshan 528400)

机构地区：[1]广东省中山市中医院泌尿外科,广东中山528400

出　　处：《湖北中医药大学学报》2021年第3期26-29,共4页Journal of Hubei University of Chinese Medicine

基　　金：广东省中山市社会公益科技研究项目(项目编号:2019B1042);广东省中医药局科研项目(项目编号:20201378)。

摘　　要：目的系统观察MicroRNA-188(微小RNA-188,miR-188)对前列腺癌细胞增殖/凋亡的调控作用以及自拟固本散结方对其干预机制。方法本研究分为两个部分:(1)将前列腺癌症穿刺阳性患者与前列腺增生切除术后病理良性患者各50例为研究对象,比较两组患者miR-188及细胞相关凋亡因子蛋白细胞增殖核抗原(Ki-67)、凋亡相关因子半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)、血清B淋巴细胞瘤基因-2相关X蛋白(Bax)、B淋巴细胞瘤基因-2蛋白(Bcl-2)的表达量差异。(2)将100例前列腺癌患者遵循随机抽样原则分为对照组与治疗组各50例,对照组行单纯西医标准内分泌治疗,治疗组行固本散结方药联合西医标准内分泌治疗,通过比较两组细胞mi R-188及相关凋亡因子Ki-67、Caspase-3、Bax、Bcl-2的表达量差异,观察自拟固本散结方的作用机制。结果(1)与前列腺增生切除术后病理良性患者比较,前列腺癌症穿刺阳性患者miR-188表达显著上调,细胞相关凋亡因子Ki-67、Bcl-2的表达量显著上升,Caspase-3和Bax表达量显著下降(P<0.05);(2)与对照组比较,观察组miR-188表达显著下调,细胞相关凋亡因子Ki-67、Bcl-2的表达量显著降低,Caspase-3和Bax的表达量显著上升(P<0.05)。结论在前列腺癌患者中,miR-188的高表达通过对细胞相关凋亡因子Ki-67、Caspase-3、Bax、Bcl-2进行表达调控以促进前列腺癌细胞的增殖。而自拟固本散结方药联合西医标准内分泌治疗能通过下调mi R-188的表达,促进Caspase-3和Bax表达,抑制Ki-67、Bcl-2表达,对前列腺癌细胞具有明显的抑制增殖、诱导凋亡的作用。Objective Treatment of prostate cancer by systematically exploring the regulatory effect of Micro RNA-188(mi R-188)on the proliferation/apoptosis of prostate cancer cells and the intervention mechanism of Guben Sanjie Formula.Methods(1)50 cases of patients with prostate cancer positive puncture and 50 cases of patients with benign prostatic hyperplasia after prostatectomy were selected as the study objects.The expression of mi R-188,Ki-67,Caspase-3,Bax and Bcl-2 in the two groups were compared.(2)100 cases of patients with prostate cancer were divided into control group and treatment group according to the principle of random sampling,50 cases each.Control group was treated with western medicine standard endocrine therapy alone,and the treatment group was treated with Guben Sanjie Formula combined with western medicine standard endocrine therapy.By comparing the expression of mi R-188,Ki-67,Caspase-3,Bax and Bcl-2 between the two groups,the mechanism of Guben Sanjie Formula was observed.Results(1)The expression of Ki-67,Bcl-2,Caspase-3 and Bax in patients with benign prostatic hyperplasia after prostatectomy was significantly lower than that in patients with positive prostate cancer puncture(P<0.05).(2)The expression of Ki-67,Bcl-2,Caspase-3 and Bax in patients with standard endocrine therapy was significantly decreased,and the expression of Caspase-3 and Bax was significantly increased(P<0.05).Conclusion In prostate cancer patients,mi R-188 can promote the proliferation of prostate cancer cells by regulating the expression of Ki-67,Caspase-3,Bax,Bcl-2 and so on.The combination of Guben Sanjie Formula and western medicine standard endocrine therapy can decline the expression of mi R-188,then promote the expression of Caspase-3 and Bax,inhibit the expression of Ki-67 and Bcl-2,and obviously inhibit the proliferation and apoptosis of prostate cancer cells.

关 键 词：前列腺癌 微小RNA-188 固本散结方 细胞增殖 细胞凋亡 

分 类 号：R285.6[医药卫生—中药学]