通化地区朝鲜族群29个Y-STR基因座遗传多态性及关联分析  

Genetic Polymorphism and Correlative Analysis of 29 Y-STR Loci of Korean-ethnic Population in Tonghua Area

作　　者：王敬群 丁光树 张译文[1] 齐迹[1] 莫晓婷[1] 李万水[1] 赵兴春[1] 叶健[1] 张建[1,2] WANG Jingqun;DING Guangshu;ZHANG Yiwen;QI Ji;MO Xiaoting;LI Wanshui;ZHAO Xingchun;YE Jian;ZHANG Jian(Institute of Forensic Science,Ministry of Public Security&Beijing Engineering Research Center of Crime Scene Evidence Examination&National Engineering Laboratory for Forensic Science,Beijing 100038,China;Xiʼan Jiaotong University Xiʼan 710048,China)

机构地区：[1]公安部物证鉴定中心,北京市现场物证检验工程技术研究中心,现场物证溯源技术国家工程实验室,北京100038 [2]西安交通大学,西安710048

出　　处：《刑事技术》2021年第4期401-407,共7页Forensic Science and Technology

基　　金：“十三五”国家重点研发计划(2017YFC0803508、2017YFC0803507);公安部科技强警基础工作专项(2018GABJC13);中央级公益性科研院所基本科研业务费专项资金项目(2020JB006)。

摘　　要：目的研究通化地区朝鲜族人群29个Y-STR(Y chromosomal short tandem repeats,Y-STR)基因座的遗传多态性及其与国内其他民族群体的遗传关系。方法采用DNATyper^(TM)Y29荧光复合扩增检测试剂盒,检测539名通化地区朝鲜族无关个体29个Y-STR基因座的DNA分型,计算基因频率等群体遗传学数据,与13个其他民族群体进行遗传距离比较分析。结果通化地区539名朝鲜族个体观察到228个基因和531种单倍型;单倍型多态性(haplotype diversity,HD)值为0.999940;基因多态性(gene diversity,GD)值在0.3138(DYS391)到0.9656(DYS385ab)之间;匹配概率(matching probability,MP)0.001917,系统鉴别能力(discrimination capacity,DC)值0.985158。通化朝鲜族和首尔韩民族(亦称朝鲜族)人群的亲缘关系最近,与吉林满族人群的亲缘关系最远。结论所测29个Y-STR基因座在通化地区具有较好的多态性分布,本研究所得基因频率、多态性等数据,可为该地区的群体遗传学和法医学研究与应用提供基础数据参考。Objective To probe into the genetic diversity of 29 Y-STR loci of Korean-ethnic population in Tonghua area(of China’s Jilin province)and the genetic relationship with other ethnic groups in China.Methods Fluorescent DNATyper^(TM)Y29 direct amplification kit was adopted to detect 539 Korean-ethnic unrelated individuals in Tonghua area,having the 29 Y-STR loci genotypes obtained so that the genetic data,e.g.,gene frequency,were calculated and the genetic distance was comparatively measured against China’s other 13 ethnic groups.Results There were 219 genes and 531 haplotypes being observed from the tested 539 Korean-ethnic individuals.The haplotype diversity(HD)value was 0.999940.The gene diversity(GD)ranged from 0.9656(DYS385ab)to 0.3138(DYS391),with the matching probability(MP)being 0.001917 and the discrimination capacity(DC)0.985158.The closest genetic relationship showed present between the Tonghua Korean-ethnic population and the same people in Korea’s Soul,with the farthest one occurring to Jilin’s Manchu population.Conclusions The here-tested 29 Y-STR loci revealed a high polymorphic distribution among the Korean-ethnic population in Tonghua area.Both the gene frequencies and polymorphism statistics obtained with this survey can provide basic data for population genetics and/or forensic research and application in that region.

关 键 词：法医遗传学 遗传多样性 通化朝鲜族 Y-STR基因座 DNATyperTMY29试剂盒 

分 类 号：DF795.2[医药卫生—法医学]