溆浦鹅LEPR基因的克隆及表达分析  

Cloning and Expression Analysis of LEPR Gene in Xupu Goose(Anser cygnoides)

作　　者：曲湘勇[1,2] 柳序 邓雨英 程浩 郭松长 贺长青[1,2] QU Xiang-Yong;LIU Xu;DENG Yu-Ying;CHENG Hao;GUO Song-Chang;HE Chang-Qing(College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;Hunan Collaborative Innovation Center of Animal Production Safety,Changsha 410128,China)

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]湖南省畜禽安全生产协同创新中心,长沙410128

出　　处：《农业生物技术学报》2021年第7期1313-1321,共9页Journal of Agricultural Biotechnology

基　　金：湖南省教育厅(自科)重点项目(18A089);湖南省重点研发计划—农业领域技术创新项目(2016NK2106)。

摘　　要：瘦素受体(leptin receptor,LEPR)是Ⅰ型细胞因子受体超家族的成员,在哺乳动物的采食、能量平衡和繁殖中起关键作用。溆浦鹅(Anser cygnoides)是湖南省优良的地方鹅品种,其肥肝性能优异,具有较高的营养价值。为了探究LEPR基因在溆浦鹅不同组织中的表达特性,本研究采用RT-PCR方法克隆了溆浦鹅LEPR基因(GenBank No.MK055330)全编码序列,并运用生物软件对LEPR蛋白空间结构进行分析。同时采用实时荧光定量PCR对LEPR基因在溆浦鹅不同组织中的表达模式以及LEPR基因在脂肪组织中表达量与肝重、肝体比和血液生化指标的相关性进行了初步研究。结果表明:溆浦鹅LEPR基因CDS区长度为3468 bp,编码1155个氨基酸,与其他禽类动物LEPR基因具有较高同源性;系统进化分析发现,溆浦鹅与浙东白鹅(Zhedong white goose)的亲缘关系最近,其次是鸭(Anas platyrhynchos)和鸡(Gallus gallus),与哺乳动物的亲缘关系较远。LEPR基因在溆浦鹅8个组织中均有表达,表达量由高到低依次为睾丸、脾脏、皮脂、肝脏、腹脂、心脏、腿肌、胸肌。LEPR基因在脂肪组织中的表达量与肝重和谷草转氨酶(aspartate aminotransferase,AST)含量呈显著负相关。本研究可为肝用型鹅的分子遗传研究提供候选基因,为LEPR基因的生物学功能研究提供基础资料。Leptin receptor(LEPR), a member of the classⅠ cytokine receptor superfamily, plays a key role in feed intake, energy balance and reproduction in mammals. Xupu goose(Anser cygnoides), an excellent local goose breed in Hunan Province, has excellent fatty liver performance and high nutritional value. To investigate the expression of LEPR at different tissues of Xupu goose, in this study, the coding sequence of LEPR was cloned by RT-PCR, and the putative protein spatial structure of LEPR was analyzed by biology software.Additionally, the expression of LEPR in Xupu goose different tissues and the correlation between LEPR expressions of adipose tissues and liver weight, ratio of liver to body weight, and blood biochemical indices were tested by quantitative Real-time PCR(qRT-PCR). The results showed that the coding sequence of LEPR was 3 468 bp, encoding 1 155 amino acids, which had high homology with LEPR gene of other poultry.phylogenetic analysis showed that Xupu goose had the closest genetic relationship with Zhedong white goose,followed by duck(Anas platyrhynchos) and chicken(Gallus gallus), and had a distant genetic relationship with mammals. LEPR gene was expressed in eight tissues of Xupu goose, including testis, spleen, sebum, liver,abdominal fat, heart, leg muscle, and chest muscle. The expression of LEPR gene in adipose tissue was negatively correlated with liver weight and aspartate aminotransferase(AST) content. This study provides a candidate gene for the molecular genetic study of liver type goose, and further provides a basis for studying the biological function of LEPR gene.

关 键 词：瘦素受体基因(LEPR) 基因克隆 溆浦鹅 表达分析 

分 类 号：S835[农业科学—畜牧学]