LEPR基因InDel检测及其与同羊尾脂和生长性状关联分析  

InDel Detection of LEPR Gene and Its Association Analysis with Tail Fat and Growth Traits of Tong Sheep(Ovis aries)

作　　者：易晓华 刘世荣 张宇[1] 潘赟 袁婷婷 孙秀柱[1,2] YI Xiao-Hua;LIU Shi-Rong;ZHANG Yu;PAN Yun;YUAN Ting-Ting;SUN Xiu-Zhu(College of Animal Science and Technology,Northwest A&F University,Tangling 712100,China;College of Grassland Agriculture,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]西北农林科技大学草业与草原学院,杨凌712100

出　　处：《农业生物技术学报》2021年第6期1142-1149,共8页Journal of Agricultural Biotechnology

基　　金：农业农村部“农牧交错带牛羊牧繁农育关键技术集成示范项目”(16190050-10);陕西省重点研发计划(2018ZDXM-NY-040)。

摘　　要：同羊是我国著名的肉毛兼用脂尾半细毛地方绵羊(Ovis aries)品种,脂肪组织是储存能量、为机体供应能量的器官,可分泌瘦素(leptin,LEP)、瘦素受体(leptin receptor,LEPR)和炎症因子等。脂肪细胞因子信号通路(adipocytokine)中LEP和LEPR基因可以协同调节脂肪沉积和能量代谢。本研究为了验证LEPR基因的插入/缺失(insertion/deletion,InDels)变异,对同羊尾脂性状及生长性状的影响,通过Ensemble数据库筛选LEPR基因的InDel(插入/缺失)位点,根据数据库中筛选的InDels位点所在的序列设计引物,鉴定并分析了166只同羊健康个体。关联分析表明:LEPR基因17 bp InDel多态位点对同羊尾宽与尾长性状的影响均不显著(P>0.05),说明该位点对尾脂性状影响较小;该位点DD基因型公羊的胸深相对于ID基因型是显著升高的(P>0.05),在公羊体高、背高和荐高等生长性状上,DD基因型显著高于II基因型(P<0.05),该InDel位点II基因型母羊腰角宽显著高于DD基因型(P<0.05),表明该位点对部分生长性状有显著影响。以上结果为LEPR基因在同羊尾型的选育以及同羊的选种选育提供了理论依据,为同羊遗传育种改良提供重要参考。Tong sheep is a well-known local sheep(Ovis aries)with fat tail and semi-fine wool for both meat and wool.Adipose tissue is an organ that stores energy and supplies energy to the body.Adipose tissue could secrete leptin(LEP),leptin receptor(LEPR),inflammatory factors and so on..The LEP and LEPR genes in the adipocytokine signaling pathway can coordinately regulate fat deposition and energy metabolism.In this study,in order to verify the insertion/deletion(InDels)variation of the LEPR gene and its influence on the fat traits and growth traits of the sheep tail,InDel locus of the LEPR gene was screened through the Ensemble database,and primers were designed according to the sequence of Indels locus screened in the database.166 healthy individuals of Tong sheep were identified and analyzed.Association analysis showed that the 17 bp InDel polymorphism of LEPR gene had no significant effect on tail width and tail length traits of the same sheep(P>0.05),indicating that this locus had little effect on tail fat traits;The breast depth of DD genotype at this locus was significantly higher than that of ID genotype(P>0.05).The DD genotype was significantly higher than that of the II genotype in the growth traits of ram body height,back height and recommended height(P<0.05).The ewe waist width of the II genotype at InDel locus was the significantly higher than that of the DD genotype(P<0.05),indicating that this locus had a significant impact on some growth traits.These results provide a theoretical basis for the selection of the LEPR gene in tail type of Tong sheep and the selection and breeding of the Tong sheep,and provide an important reference for the genetic breeding and improvement of the Tong sheep.

关 键 词：瘦素受体(LEPR) 同羊 插入/缺失(InDels) 尾脂 生长性状 

分 类 号：S813.3[农业科学—畜牧学]