福建省猪源BVDV流行病学检测分析及其对CSFV抗体的影响  

Epidemiological detection and analysis of bovine viral diarrhea virus(BVDV)from swine and its effect on CSFV antibody in Fujian Province

作　　者：徐磊[1] 曾亮明[2,3] 张体银 刘毅发 杨慧 余勋信[1] 林秋敏[1] 刘小龙[5] 傅光华 白泉阳[4] 黄瑜 张渊魁[3,6] XU Lei;ZENG Liangming;ZHANG Tiyin;LIU Yifa;YANG Hui;YU Xunxin;LIN Qiumin;LIU Xiaolong;FU Guanghua;BAI Quanyang;HUANG Yu;ZHANG Yuankui(Fujian Agricultural Vocational Technical College,Fuzhou 350119,China;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Zhaofenghua Biotechnology(Fuzhou)Co.,Ltd.,Fuzhou 350014,China;Technology Center of Fuzhou Customs,Fuzhou 350001,China;Present(Fuzhou)Biotech Company Limited,Fuzhou 350500,China;Zhaofenghua Biotechnology(Nanjing)Co.,Ltd.,Nanjing 211102,China)

机构地区：[1]福建农业职业技术学院,福建福州350119 [2]福建省农业科学院畜牧兽医研究所,福建福州350013 [3]兆丰华生物科技(福州)有限公司,福建福州350014 [4]福州海关技术中心,福建福州350001 [5]派生特(福州)生物科技有限公司,福建福州350500 [6]兆丰华生物科技(南京)有限公司,江苏南京211102

出　　处：《中国兽医学报》2021年第6期1031-1037,1059,共8页Chinese Journal of Veterinary Science

基　　金：福建省第七批引才“百人计划”资助项目;2019年度福建省科技计划资助项目(2019N0025);2019年度江苏省博士后科研资助计划资助项目(2019K072);2018年福建省中青年教师教育科研资助项目(JZ180531);2020年度福州市科技计划资助项目(2020-PT-149);福建省畜禽疫病防控技术重大研发平台资助项目(2014N2003);福建省高层次人才和青年优秀人才访学研修资助计划资助项目(闽人社文[2019]239号);福建省职业院校专业带头人培养计划资助项目(闽教办师[2019]40号)。

摘　　要：为了解福建省猪源牛病毒性腹泻病毒(BVDV)流行与分布情况及对猪瘟病毒(CSFV)抗体的影响,分别采用已有ELISA方法和间接血凝试验(IHA)对福建省9个地市293个猪场的16537份临床样品进行猪源BVDV抗体和CSFV IHA抗体效价(log2x)进行检测,并对其中400份样品采用已有RT-PCR方法开展猪源BVDV病原检测及测序。结果显示16537临床样品、293个猪场和9个地市的BVDV抗体阳性率分别为35.6%(5891/16537),89.4%(262/293)和100.0%(9/9),其中母猪和疑似猪瘟猪的BVDV抗体阳性率分别显著高于仔猪和临床健康猪(P<0.05);BVDV抗体阴性猪的CSFV IHA抗体效价与阳性率均显著高于BVDV抗体阳性猪(P<0.05);CSFV IHA抗体效价在6~11猪群的BVDV抗体多为阴性,占比95.9%(8479/8842);BVDV抗体阳性猪群的CSFV IHA抗体效价普遍较低(≤4),占比88.7%(5224/5891);且CSFV IHA抗体与BVDV抗体呈负相关(r=-0.819,P<0.05);BVDV病原阳性率为15.3%(61/400),其中抗体-/抗原-占45.0%(180/400),抗体+/抗原-占39.8%(159/400)、抗体-/抗原+占12.0%(48/400),抗体+/抗原+占3.2%(13/400)。结果表明,福建省猪源BVDV感染情况较普遍并对CSFV抗体存在抑制作用,母猪与疑似猪瘟猪为BVDV感染的高流行群体,对抗体-/抗原+持续感染猪应予以重视。This study aims to investigate the prevalence and distribution of bovine viral diarrhea virus(BVDV)in swine in Fujian Province and its effect on classical swine fever(CSF)virus antibody.The BVDV antibody and CSF virus(CSFV)indirect hemagglutination assay(IHA)antibody(log2x)of 16537clinical samples from 293swinefarms in 9regions of Fujian province were detected by the developed ELISA and IHA respectively.And 400samples from them were detected by the developed RT-PCR and sequenced.The results showed that the BVDV antibody positive rate in samples,swine farms and 9regions were 35.6%(5891/16537),89.4%(262/293)and 100.0%(9/9)respectively.Among them,the BVDV antibody positive rate in sow and swine suspected of CSF were significantly higher than that in piglet and clinical healthy swine(P<0.05),and the titer and positive rate of CSFV IHA antibody in BVDV antibody negative swine were significantly higher than those in BVDV antibody positive swine(P<0.05).The BVDV antibody of swines with CSFV IHA antibody ranged from 6to 11were mostly negative,accounted for 95.9%(8479/8842).The CSFV IHA antibody of BVDV antibody positive swine were generally low(≤4),accounted for 88.7%(5224/5891).There was a negative correlation between the CSFV IHA antibody and BVDV antibody(r=-0.819,P<0.05).In addition,the BVDV positive rate of swine was 15.3%(61/400).Among them,antibody-/antigen-,antibody+/antigen-,antibody-/antigen+,antibody+/antigen+were accounted for 45.0%(180/400),39.8%(159/400),12.0%(48/400)and 3.2%(13/400)respectively.These findings indicated that the infection of swine BVDV was common in Fujian Province and had inhibitory effect on CSFV antibody.Sow and swine suspected of CSF were highly prevalent groups of BVDV infection,and attention should be paid to antibody-/antigen+persistent infection in swine.

关 键 词：福建省 猪源牛病毒性腹泻病毒 流行病学 猪瘟病毒抗体 抑制作用 

分 类 号：S855.3[农业科学—临床兽医学]