自体外周血来源的PBMC诱导分化为iPSCs结合NGF/Coll-H复合支架修复兔喉返神经损伤  

Exploration of the effect of autologous peripheral blood derived PBMC differentiation into iPSCs combined with NGF/coll-h composite scaffold on repair of rabbit's recurrent laryngeal nerve injury

作　　者：潘永勤[1] 马征来 李进义[1] PAN Yong-qin;MA Zheng-lai;LI Jin-yi(Department of Thyroid Surgery,the First Affiliated Hospital of Jinan University,Guangzhou 510630,China)

机构地区：[1]暨南大学附属第一医院甲状腺外科,广东广州510630 [2]山东省临沂市妇女儿童医院,山东临沂371311

出　　处：《解剖学研究》2021年第4期302-306,共5页Anatomy Research

基　　金：广东省医学科研基金项目(A2019190);广州市科技计划项目(202102010120)。

摘　　要：目的利用对自体外周血来源的PBMC进行重编程为诱导多能干细胞(iPSCs),探讨在此基础上与NGF/Coll-H复合支架相结合修复兔喉返神经损伤的可能性。方法新西兰大白兔随机分为5组:喉返神经单纯端-端吻合组、神经端端吻合-Coll-H支架治疗组、端端吻合-Coll-H-NGF治疗组、端端吻合-Coll-H-NGF-iPSCs治疗组以及假手术组。对比分析复合支架植入到喉返神经损伤部位的修复效果。结果多项指标表明iPSCs/NGF/Coll-H复合支架的修复效果要显著高于其他组。结论自体外周血来源的PBMC诱导的iPSCs与NGF/Coll-H复合支架相结合更有利于治疗喉返神经手术带来的损伤。Objective To reprogram PBMCs derived from autologous peripheral blood into iPSCs,combined with NGF/Coll-H composite scaffold to treat recurrent laryngeal nerve injury.Methods In this study,rabbit were randomly divided into 5 groups:simple end-to-end anastomosis group,end-to-end anastomosis-Coll-H scaffold group,end-to-end anasto-mosis-Coll-H-NGF group,end-to-end anastomosis-Coll-H-NGF-iPSCs group and sham operation group.We compared and analyzed the repair effect of composite stent implantation on the site of recurrent laryngeal nerve injury.Results The repair effect of iPSCs/NGF/Coll-H composite scaffold group was significantly higher than that of the other groups.Conclusion The combination of induced pluripotent stem cells(iPSCs)derived from autologous peripheral blood and NGF/Coll-H composite scaffold was more conducive to the treatment of recurrent laryngeal nerve injury.

关 键 词：喉返神经损伤 诱导多能干细胞 iPSCs/NGF/Coll-H支架 PBMC 兔 

分 类 号：R651.3[医药卫生—外科学]