Protein farnesylation negatively regulatesbrassinosteroid signaling via reducing BES1 stabilityinArabidopsis thaliana^(∞)  

作　　者：Zengxiu Feng Hongyong Shi Minghui Lv Yuang Ma Jia Li 

机构地区：[1]Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations,School of Life Sciences,Lanzhou University,Lanzhou 730000,China

出　　处：《Journal of Integrative Plant Biology》2021年第7期1353-1366,共14页植物学报（英文版）

基　　金：supported by the National Natural Sci-ence Foundation of China Grants 31720103902 ,32030005(to J.L.);111 Project B16022(to J.L.);the Funda-mental Research Funds for the Central Universities Grant no.lzujbky‐2020‐sp04(to J.L.),lzujbky‐2021‐kb05。

摘　　要：Brassinosteroids(BRs)are a group of steroidal phytohormones,playing critical roles in almost all physiological aspects during the life span of a plant.In Arabidopsis,BRs are perceived at the cell surface,triggering a reversible phosphorylation-based signaling cascade that leads to the activation and nuclear accumulation of a family of transcription factors,represented by BES1 and BZR1.Protein famesylation is a type of post-translational modification,functioning in many important cellular processes.Previous studies demonstrated a role of famesylation in BR biosynthesis via regulating the endoplasmic reticulum localization of a key bassino-lide(BL)biosynthetic enzyme BR6ox2.Whether such a process is also involved in BR signaling is not understood.Here,we demonstrate that protein famesylation is involved in mediating BR signaling in Arabidopsis.A loss-of-function mutant of EN HANCED RESPONSE TO ABA 1(iERA1).encoding a(3 subunit of the protein famesyl transferase hoi oenzyme,can alter the BL sensitivity of bak1~4 from a reduced to a hypersensitive level,era 7 can partially rescue the BR defective phenotype of a hetero zygous mutant of bin2-1,a gain-of function mutant of BIN2 which encodes a negative regulator in the BR signaling.Our genetic and biochemical analyses revealed that ERA1 plays a significant role in regulating the protein stability of BES1.

关 键 词：Arabidopsis BES1 BRASSINOLIDE BRASSINOSTEROIDS protein famesylation 

分 类 号：Q943[生物学—植物学]