Structure of plant photosystem I−light harvesting complex I supercomplex at 2.4Å resolution  

作　　者：Jie Wang Long-Jiang Yu Wenda Wang Qiujing Yan Tingyun Kuang Xiaochun Qin Jian-Ren Shen 

机构地区：[1]Photosynthesis Research Center,Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Beijing,100093 China [2]University of Chinese Academy of Sciences,Beijing,100049 China [3]School of Biological Science and Technology,University of Jinan,Jinan,250022 China

出　　处：《Journal of Integrative Plant Biology》2021年第7期1367-1381,共15页植物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2017YFA0503700);the Chinese Academy of Sciences Key Research Program of Frontier Sciences(QYZDY-SSW-SMC003);a Strategic Priority Research Program of CAS(XDB17000000);a National Basic Research Program of China(2015CB150100);the National Natural Science Foundation of China(31622007,31670237,32070267);the Youth Innovation Promotion Association of CAS(2020081);Taishan Scholars Project,Jinan Science and Technology Project(2020GXRC058);the Natural Science Foundation of Shandong Province China(ZR2019ZD48).

摘　　要：Photosystem I(PSI)is one of the two photosystems in photosynthesis,and performs a series of electron transfer reactions leading to the reduction of ferredoxin.In higher plants,PSI is surrounded by four light-harvesting complex I(LHCI)subunits,which harvest and transfer energy efficiently to the PSI core.The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6Åresolution,providing much information on the arrangement of proteins and cofactors in this complicated supercomplex.Here we have optimized crystallization conditions,and analyzed the crystal structure of PSI-LHCI at 2.4Åresolution.Our structure showed some shift of the LHCI,especially the Lhca4 subunit,away from the PSI core,suggesting the indirect connection and inefficiency of energy transfer from this Lhca subunit to the PSI core.We identified five new lipids in the structure,most of them are located in the gap region between the Lhca subunits and the PSI core.These lipid molecules may play important roles in binding of the Lhca subunits to the core,as well as in the assembly of the supercomplex.The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting,transfer and assembly of this supercomplex.

关 键 词：crystal structure Lhca LHCI LIGHT-HARVESTING PEA PHOTOSYNTHESIS photosystem I 

分 类 号：Q945[生物学—植物学]