新型冠状病毒肺炎合并动脉硬化患者的肠道菌群分析  

Analysis of the intestinal flora in novel coronavirus pneumonia patients with arterial stiffness

作　　者：魏秀先 梁小璐 廖鑫[1] 余维巍[1] 张存泰[1] 糜涛[1] Wei Xiuxian;Liang Xiaolu;Liao Xin;Yu Weiwei;Zhang Cuntai;Mi Tao(Department of Geriatrics,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China)

机构地区：[1]华中科技大学同济医学院附属同济医院综合医疗科,武汉430000

出　　处：《中华老年医学杂志》2021年第7期890-894,共5页Chinese Journal of Geriatrics

基　　金：国家重点研发计划项目(2017YFD0400304);湖北省技术创新专项重大项目(2019ACA141)。

摘　　要：目的分析新型冠状病毒肺炎合并动脉硬化患者的肠道菌群组成及功能特征,为临床合理使用肠道微生态调节剂提供理论依据.方法招募2020年4月5日至4月19日之间收住在我院的新型冠状病毒肺炎患者为研究对象,按动脉硬化检测结果分为硬化组和对照组.入院7d内采集粪便样本,提取肠道菌群DNA并建库,采用鸟枪法进行宏基因测序,基于测序结果进行生物信息学分析.结果本研究共纳入16例新型冠状病毒肺炎患者,硬化组7例,对照组9例.硬化组baPWV值及血压值高于对照组(P<0.05).肠道菌群物种分析结果:门水平beta多样性分析显示组间肠菌群落构成差异明显(P<0.05).与对照组相比,硬化组患者粪便微生物中Holdemanella、Mitsuokella、Deinococcus、Lachnospira、Turicibacter、Butyrivibrio、Sporomusa、Halanaerobium相对丰度显著降低,这些菌群与产生SCFAs、调节能量代谢、抗辐射、抗氧化应激和抗炎作用相关.KEGG通路富集分析结果:对照组菌群功能主要富集在脂多糖生物合成、短链脂肪酸代谢、其他氨基酸代谢及膜运输等通路上,硬化组主要富集在氨基酸代谢和DNA损伤修复通路上.结论新型冠状病毒感染后,患者使用增加短链脂肪酸的微生物制剂可能有助于恢复肠道菌群稳态.Objective To analyze the composition and functional characteristics of the intestinal microflora in novel coronavirus pneumonia patients with arterial stiffness,in order to provide empirical evidence for rational use of intestinal microecological modulators.Methods Patients with novel coronavirus pneumonia admitted to our hospital between April 5 and April 19,2020 were enrolled as research subjects.They were divided into the stiffness group and the control group according to the results of arterial stiffness.Stool samples were collected within 7 days of admission.Intestinal flora DNA was analyzed and entered into a database,shotgun metagenomic sequencing was performed,and bioinformatics analysis was conducted based on sequencing results.Results A total of 16 patients with novel coronavirus pneumonia were included in this study,including 7 in the stiffness group and 9 in the control group.Brachial-ankle pulse wave velocity and blood pressure were higher in the stiffness group than in the control group(P<0.05).Beta diversity analysis at the phylum level showed that there were significant differences in the composition of the intestinal flora between the two groups(P<0.05).Patients with arterial stiffness had a lower relative abundance than the controls in fecal Holdemanella,Mitsuokella,Deinococcus,Lachnospira,Turicibacter,Butyrivibrio,Sporomusa,and Halanaerobium,species associated with the production of short-chain fatty acids(SCFAs),regulation of energy metabolism,anti-radiation,anti-oxidative stress and anti-inflammatory effects.The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis showed that the intestinal flora in the control group was mainly involved in the pathways of lipopolysaccharide biosynthesis,metabolism of SCFA and other amino acids,and membrane transport,while the intestinal flora in the stiffness group was mostly concerned with the pathways of amino acid metabolism and DNA damage repair.Conclusions The use o£microbial agents capable of increasing short-chain fatty acids in pa

关 键 词：肺炎 病毒感染 动脉硬化 肠道菌群 

分 类 号：R563.1[医药卫生—呼吸系统] R543.5[医药卫生—内科学]