杞藿饮对运动性疲劳大鼠肌组织保护作用及其对大鼠行为学改变的影响  

Protective effect of Qihuo Yin on muscle tissue of exercise-induced fatigue rats and its influence on behavioral changes of rats

作　　者：王学芳 王鹏[1] 封颖璐 WANG Xue-fang;WANG Peng;FENG Ying-lu(School of Basic Medicine,Qingdao University,Qingdao 266071,China;Department of Traditional Chinese Medicine,Navy No.971 Hospital of the People’s Liberation Army,Qingdao 266071)

机构地区：[1]青岛大学基础医学院,青岛266071 [2]中国人民解放军海军第971医院中医科,青岛266071

出　　处：《北京中医药》2021年第5期482-485,共4页Beijing Journal of Traditional Chinese Medicine

基　　金：全军科技青年培育计划(18QNP013);青岛市医疗卫生优秀人才培养项目。

摘　　要：目的观察杞藿饮对运动性疲劳大鼠肌组织的保护作用及其对大鼠行为学改变的影响,探讨其抗疲劳作用机制。方法雄性SD大鼠18只,随机分为空白对照组、模型组、杞藿饮组,每组6只。采用跑台运动建立大鼠力竭运动疲劳模型。空白对照组与模型组均给予等体积的生理盐水灌胃,杞藿饮组给予杞藿饮灌胃,1次/d,连续7 d;空白对照组不给予任何训练,模型组与杞藿饮组每天灌胃30 min后给予跑台训练30 min(1530 m/min),连续6 d,于第7 d进行速度为30 m/min的一次性力竭跑台运动。跑台结束后记录力竭运动时间,检测大鼠血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、乳酸(LA)、尿素氮(BUN)含量的变化并观察大鼠行为学变化。结果与空白对照组相比,模型组大鼠血清LDH、LA、CK、BUN水平均明显升高(P<0.01)。与模型组相比,杞藿饮组大鼠的力竭运动时间明显延长(P<0.01),且血清LDH、LA、CK、BUN水平均明显降低(P<0.05);杞藿饮组大鼠转棒实验动物潜伏期无明显变化,但自主活动反应箱总区域活动潜伏期明显延长(P<0.05)。结论杞藿饮可明显延长大鼠力竭运动时间并对大鼠自主活动有一定的影响,可能是通过减少大鼠运动疲劳时的多种代谢产物的产生,从而发挥抗疲劳的作用。Objective To observe the protective effect of Qihuo Yin on muscle tissue of rats after acute exhaustive exercise and its influence on behavioral changes,and to explore its anti-fatigue mechanism.Methods Eighteen male Sprague-Dawley rats were randomly divided into blank control group,model group and the Qihuo Yin group,with 6 rats in each group.Treadmill exercise was used to establish exhaustive exercise fatigue model of rats.The blank control group was given the same volume of normal saline by gavage once a day as the rats in the model group,and the rats in the Qihuo Yin group were given Qihuo Yin by gavage once a day;the course of treatment was 7 consecutive days for all groups.The blank control group were not given any training.The rats in the model group and the Qihuo Yin group were given treadmill training for 30 minutes(15-30 m/min)30 minutes after gavage for 6 consecutive days,and on day7,they were given treadmill training with a speed of 30 m/min.Exhaustive exercise time was recorded for the rats in the model group and the Qihuo Yin,and the serum levels of creatine kinase(CK),lactate dehydrogenase(LDH),lactic acid(LA),urea nitrogen(BUN)were measure.At the same time,the behavioral changes of rats were observed.Results Compared with the blank control group,the levels of serum LDH,LA,CK,BUN in the model group were significantly higher than those in the blank control group(P<0.01).Compared with the model group,the exhaustive exercise time of rats in Qihuo Yin group was significantly longer than that in the model group(P<0.01),and the levels of serum LDH,LA,CK,BUN were significantly lower than those in the model group(P<0.05);there was no significant change in the incubation period of the rats rotating rod test animals in the Qihuo Yin group,but the activity latency of the total area of the autonomic activity reaction box was significantly prolonged(P<0.05).Conclusion Qihuo Yin can significantly prolong the exhaustive exercise time of rats and have an effect on the autonomic activity of rats.It plays an anti-fa

关 键 词：运动性疲劳 杞藿饮 跑台 行为学 大鼠 

分 类 号：R285.5[医药卫生—中药学]