维生素D受体基因Bg1I、Cdx-2位点多态性与桥本氏甲状腺炎的相关性  

The Correlation between Vitamin D Receptor Gene Bg1I and Cdx-2 Polymorphism and Hashimoto's Thyroiditis

作　　者：阮小荟 向茜[1] 王玉明[2] 周治含 张弦[1] 郭燕[1] 杨晓瑞 RUAN Xiao-hui;XIANG Qian;WANG Yu-ming;ZHOU Zhi-han;ZHANG Xian;GUO Yan;YANG Xiao-rui(Dept.of Endocrinology,The 5th Affiliated Hospital of Kunming Medical University Yunnan/Honghe South Yunnan Central Hospital,Gejiu Yunnan 661000;Dept.of Clinical Laboratory,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Dept.of Nuclear Medicine,The 5th Affiliated Hospital of Kunming Medical University Yunnan/Honghe South Central Hospital Gejiu,Gejiu Yunnan 661000,China)

机构地区：[1]昆明医科大学第五附属医院(红河州滇南中心医院)内分泌科,云南个旧661000 [2]昆明医科大学第二附属医院检验科,云南昆明650101 [3]昆明医科大学第五附属医院(红河州滇南中心医院)核医学科,云南个旧661000

出　　处：《昆明医科大学学报》2021年第8期132-139,共8页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2018JS255);云南省卫生健康委医学学科带头人培养计划基金资助项目(D-2018034)。

摘　　要：目的探讨VDR基因Bg1I、Cdx-2多态性与桥本氏甲状腺炎(hashimoto’sthyroiditis,HT)的相关性。方法应用TaqMan荧光探针技术,对178例HT组,其中甲功正常(H0)组56例,亚临床甲减(H1)组80例,甲减(H2)组42例和64例健康对照者(NT)组的VDR基因Bg1I、Cdx-2位点SNP进行检测,比较和分析各组间基因型频率、等位基因频率以及相关临床资料。结果 (1)Bg1I位点GG、GT、TT基因型分布频率分别为0.500,0.405和0.095,等位基因G、T分布频率分别为0.702 5和0.297 5;Cdx-2位点GG、GA、AA基因型分布频率分别为0.293 4,0.528 9和0.177 7,等位基因G及A分布频率分别为0.557 9及0.4421;(2)NT及HT组间各等位基因频率及基因型分布,差异无统计学意义(P> 0.05);(3)Logistic回归分析表明25(OH)D缺乏可能是HT发生的独立危险因素(OR=1.573,P=0.046);(4)男性(OR=0.158,P <0.001)及Cdx-2位点G等位基因(OR=0.301,P=0.035)可能是HT患者发生甲减的保护因素,高滴度TPO-Ab(OR=1.639,P=0.045)及TGAb(OR=1.779,P=0.007)可能是其危险因素。结论 (1)VDR Bg1I、Cdx-2位点存在单核苷酸多态性。(2)Cdx-2位点G等位基因可能是HT发生甲减的保护因素;(3)Bg1I位点SNP与HT的发生及进展无关。Objective To investigate the correlation between Vitamin D receptor gene Bg1I, Cdx-2 polymorphism with Hashimoto’s thyroiditis(HT). Methods The SNP of VDR gene Bg1I and CDX-2 in 178 HT patients were detected by TaqMan fluorescent probe,including 56 patients with normal thyroid function(H0 group), 80 patients with subclinical hypothyroidism( H1 group), 42 patients with hypothyroidism( H2 group) and 64 healthy controls(NT group). The genotype frequency,allele frequency and related clinical data were compared and analyzed. Results Bg1I GG, GT and TT genotypes frequencies were 0.500, 0.405 and 0.095, respectively;alleles G and T were 0.702 5 and 0.297 5, respectively. CDX-2 GG, GA and AA genotypes frequencies were0.293 4,0.528 9 and 0.177 7,respectively;alleles G and A frequencies were 0.557 9 and 0.442 1,respectively.The genotype frequency and allele frequency in two groups were not significantly different( P > 0.05). Logistic regression analysis indicated 25(OH) D deficiency might be an independent risk factor for HT(OR = 1.573,P =0.046);male(OR = 0.158,P < 0.001) and CDX-2G genotype(OR = 0.301,P = 0.035) may be protective factors for hypothyroidism in HT patients. High titrate TPO-Ab(OR = 1.639,P = 0.045) and TG-Ab(OR = 1.779,P =0.035) may be risk factors for hypothyroidism in HT patients. Conclusion (1)Single nucleotide polymorphisms( SNPs) were found at VDR BG1I and CDX-2 sites.( 2) CDX-2 G genotype may be a protective factor of hypothyroidism in HT.(3)Bg1I single nucleotide polymorphism was not related to the occurrence and progression of HT.

关 键 词：维生素D受体基因 单核苷酸多态性 25羟维生素D 桥本氏甲状腺炎 甲状腺功能减退症 

分 类 号：R581.4[医药卫生—内分泌]