Developing single nucleotide polymorphism markers for the identification of pineapple(Ananas comosus)germplasm  

作　　者：Lin Zhou Tracie Matsumoto Hua-Wei Tan Lyndel W Meinhardt Sue Mischke Boyi Wang Dapeng Zhang 

机构地区：[1]Sustainable Perennial Crops Laboratory,USDA-ARS,Beltsville Agricultural Research Center,Beltsville,20705,MD,USA [2]Daniel K.Inouye Pacific Basin Agricultural Research Center,USDA-ARS,Hilo,96720,HI,USA [3]College of Horticulture,Nanjing Agricultural University,Nanjing,210095,Jiangsu,China [4]Yunnan Forestry Technological College,Kunming,650224,Yunnan,China

出　　处：《Horticulture Research》2015年第1期35-46,共12页园艺研究（英文）

基　　金：This work was partially supported by the National Natural Science Foundation of China(30800884,31370688).

摘　　要：Pineapple(Ananas comosus[L.]Merr.)is the third most important tropical fruit in the world after banana and mango.As a crop with vegetative propagation,genetic redundancy is a major challenge for efficient genebank management and in breeding.Using expressed sequence tag and nucleotide sequences from public databases,we developed 213 single nucleotide polymorphism(SNP)markers and validated 96 SNPs by genotyping the United States Department of Agriculture-Agricultural Research Service pineapple germplasm collection,maintained in Hilo,Hawaii.The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection.Twenty-four groups of duplicates were detected,encompassing 130 of the total 170 A cosmos accessions.The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple.Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties.Parentage analysis further revealed that both A.comosus var.bracteatus and A.comosus var.ananassoides are likely progenitors of pineapple cultivars.However,the traditional classification of cultivated pineapple into horticultural groups(e.g.‘Cayenne’,‘Spanish’,‘Queen’)was not well supported by the present study.These SNP markers provide robust and universally comparable DNA fingerprints;thus,they can serve as an efficient genotyping tool to assist pineapple germplasm management,propagation of planting material,and pineapple cultivar protection.The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops.

关 键 词：CULTIVAR maintained COLLECTION 

分 类 号：U46[机械工程—车辆工程]