Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy  

作　　者：Lin Li Wei Guo Yinzhou Yan Seoungjun Lee Tao Wang 

机构地区：[1]Laser Processing Research Centre,School of Mechanical,Aerospace and Civil Engineering,and Photon Science Institute,The University of Manchester,Manchester M139PL,UK [2]Faculty of Medical and Human Sciences,The University of Manchester,Manchester M139PL,UK

出　　处：《Light(Science & Applications)》2013年第1期64-72,共9页光（科学与应用)（英文版）

摘　　要：Because of the small sizes of most viruses(typically 5–150 nm),standard optical microscopes,which have an optical diffraction limit of 200 nm,are not generally suitable for their direct observation.Electron microscopes usually require specimens to be placed under vacuum conditions,thus making them unsuitable for imaging live biological specimens in liquid environments.Indirect optical imaging of viruses has been made possible by the use of fluorescence optical microscopy that relies on the stimulated emission of light from the fluorescing specimens when they are excited with light of a specific wavelength,a process known as labeling or self-fluorescent emissions from certain organic materials.In this paper,we describe direct white-light optical imaging of 75-nm adenoviruses by submerged microsphere optical nanoscopy(SMON)without the use of fluorescent labeling or staining.The mechanism involved in the imaging is presented.Theoretical calculations of the imaging planes and the magnification factors have been verified by experimental results,with good agreement between theory and experiment.

关 键 词：imaging microscope OPTICAL super-resolution virus 

分 类 号：O43[机械工程—光学工程]