Single capillary oximetry and tissue ultrastructural sensing by dual-band dual-scan inverse spectroscopic optical coherence tomography  

作　　者：Rongrong Liu James A.Winkelmann Graham Spicer Yunxiao Zhu Aya Eid Guillermo A.Ameer Vadim Backman Ji Yi 

机构地区：[1]Department of Biomedical Engineering,Northwestern University,Evanston,IL 60208,USA [2]Department of Chemical and Biological Engineering,Northwestern University,Evanston,IL 60208,USA [3]Department of Medicine,Boston University School of Medicine,Boston,MA 02118,USA

出　　处：《Light(Science & Applications)》2018年第1期511-523,共13页光（科学与应用)（英文版）

基　　金：from the Evans Medical Foundation at Boston Medical Center,the National Institute of Health(R01CA173745,R01CA183101,and R01CA165309);the National Science Foundation(CBET-1240416).

摘　　要：Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo.Here we demonstrate a novel optical imaging system,dual-band dual-scan inverse spectroscopic optical coherence tomography(D2-ISOCT),that,for the first time,can simultaneously obtain the following metrics in vivo using endogenous contrast:(1)capillary-level oxygen saturation and arteriolar-level blood flow rates,oxygen delivery rates,and oxygen metabolic rates;(2)spatial characteristics of tissue structures at length scales down to 30 nm;and(3)morphological images up to 2mm in depth.To illustrate the capabilities of D2-ISOCT,we monitored alterations to capillaries and the surrounding pericapillary tissue(tissue between the capillaries)in the healing response of a mouse ear wound model.The obtained microvascular and ultrastructural metrics corroborated well with each other,showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool.

关 键 词：optical CAPILLARY INVERSE 

分 类 号：TN9[电子电信—信息与通信工程]