Simple and efficient delivery of cellimpermeable organic fluorescent probes into live cells for live-cell superresolution imaging  

作　　者：Meng Zhang Meihua Li Wenting Zhang Yubing Han Yu-Hui Zhang 

机构地区：[1]Britton Chance Center for Biomedical Photonics,Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology,Wuhan,Hubei 430074,China [2]MoE Key Laboratory for Biomedical Photonics,School of Engineering Sciences,Huazhong University of Science and Technology,Wuhan,Hubei 430074,China

出　　处：《Light(Science & Applications)》2019年第1期563-573,共11页光（科学与应用)（英文版）

基　　金：supported by the National Basic Research Program of China(Grant No.2015CB352002);the National Natural Science Foundation of China(Grant No.31770924,61827825,31600692,31470056);the Science Fund for Creative Research Group of China(Grant No.61721092);the Director Fund of the Wuhan National Laboratory for Optoelectronics.

摘　　要：Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell permeability.Here,we develop a simple but effective strategy that renders cells permeable to cell-impermeable,organic fluorescent probes by using a novel peptide vehicle,PV-1.By simple coincubation with PV-1,22 different cell-impermeable,organic fluorescent probes were efficiently delivered into live cells and specifically labeled a variety of organelles.Moreover,PV-1 can simultaneously transfer up to three different probes into live cells.By using PV-1 and these cell-impermeable fluorescent probes,we obtained multicolor,longterm,live-cell superresolution images of various organelles,which allowed us to study the dynamic interactions between them.PV-1,together with these organic fluorescent probes,will greatly broaden the applications of superresolution imaging technology in diverse live-cell studies and opens up a new avenue in the design and application of peptide vehicles.

关 键 词：RESOLUTION allowed RENDER 

分 类 号：O62[理学—有机化学]