High-contrast,fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser  

作　　者：Cihang Kong Christian Pilger Henning Hachmeister Xiaoming Wei Tom H.Cheung Cora S.W.Lai Nikki P.Lee Kevin K.Tsia Kenneth K.Y.Wong Thomas Huser 

机构地区：[1]Department of Electrical and Electronic Engineering,The University of Hong Kong,Pokfulam Road,Hong Kong,China [2]Biomolecular Photonics,Department of Physics,University of Bielefeld,Universitatsstr,25,33615 Bielefeld,Germany [3]Division of Life Science,The Hong Kong University of Science and Technology,Hong Kong,China [4]Department of Physiology,Li Ka Shing Faculty of Medicine,The University of Hong Kong,Pokfulam,Hong Kong,China [5]Department of Surgery,The University of Hong Kong,Pokfulam Road,Hong Kong,China [6]Division of Engineering and Applied Science,California Institute of Technology,1200 East California Boulevard,Pasadena,CA 91125,USA

出　　处：《Light(Science & Applications)》2020年第1期1775-1786,共12页光（科学与应用)（英文版）

基　　金：supported by the Germany/Hong Kong Joint Research Scheme sponsored by the Research Grants Council of Hong Kong;the Germany Academic Exchange Service of Germany(G-HKU708/14,DAAD-57138104);the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No.766181,project“DeLIVER”;the Research Grants Council of the Hong Kong Special Administrative Region,China(HKU 17205215,CityU T42-103/16-N,EHKU701/17,and HKU C7047-16G);the National Natural Science Foundation of China(N_HKU712/16);Innovation and Technology Fund(GHP/050/14GD);support for the Article Processing Charge by the Deutsche Forschungsgemeinschaft;the Open Access Publication Fund of Bielefeld University.

摘　　要：Coherent Raman scattering(CRS)microscopy is widely recognized as a powerful tool for tackling biomedical problems based on its chemically specific label-free contrast,high spatial and spectral resolution,and high sensitivity.However,the clinical translation of CRS imaging technologies has long been hindered by traditional solid-state lasers with environmentally sensitive operations and large footprints.Ultrafast fibre lasers can potentially overcome these shortcomings but have not yet been fully exploited for CRS imaging,as previous implementations have suffered from high intensity noise,a narrow tuning range and low power,resulting in low image qualities and slow imaging speeds.Here,we present a novel high-power self-synchronized two-colour pulsed fibre laser that achieves excellent performance in terms of intensity stability(improved by 50 dB),timing jitter(24.3 fs),average power fluctuation(<0.5%),modulation depth(>20 dB)and pulse width variation(<1.8%)over an extended wavenumber range(2700-3550 cm^(−1)).The versatility of the laser source enables,for the first time,high-contrast,fast CRS imaging without complicated noise reduction via balanced detection schemes.These capabilities are demonstrated in this work by imaging a wide range of species such as living human cells and mouse arterial tissues and performing multimodal nonlinear imaging of mouse tail,kidney and brain tissue sections by utilizing second-harmonic generation and twophoton excited fluorescence,which provides multiple optical contrast mechanisms simultaneously and maximizes the gathered information content for biological visualization and medical diagnosis.This work also establishes a general scenario for remodelling existing lasers into synchronized two-colour lasers and thus promotes a wider popularization and application of CRS imaging technologies.

关 键 词：scattering tuning FIBRE 

分 类 号：TN248[电子电信—物理电子学]