重组禽流感病毒(H5+H7)二价灭活疫苗对地方鸡的免疫效果评价  

Evaluation of the immune effect of recombinant Avian influenza virus (H5+H7) bivalent inactivated vaccine on local chickens

作　　者：周浪 王研 杨蔚 代飞燕 ZHOU Lang;WANG Yan;YANG Wei;DAI Feiyan(Dali Bai Autonomous Prefecture Animal Disease Prevention and Control Center,Dali 671000,China;College of Animal Medicine,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]大理白族自治州动物疫病预防控制中心,云南大理671000 [2]云南农业大学动物医学院,昆明650201

出　　处：《黑龙江畜牧兽医》2021年第13期86-90,148,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：朱兴全专家工作站项目(202005AF150041)。

摘　　要：为了探究国家农业农村部2017年推出的重组禽流感病毒(H5+H7)二价灭活疫苗(H5N1 Re-8株+H7N9 H7-Re1株)的免疫效果,试验根据母源抗体检测结果及抗体消长规律确定首免和二免的时间及注射剂量,对土杂鸡、芦花鸡、瑶鸡、青花鸡、珍珠鸡和武定鸡这6个品种云南地方鸡进行疫苗注射接种,采用血凝(HA)和血凝抑制(HI)试验测定免疫前9,1天、首免后7,14,21,28天及二免后7,14,21,28,35,49,63,77,91,105天的抗体效价,同时绘制抗体消长曲线,分析6个品种云南地方鸡的禽流感抗体水平并评价免疫效果。结果表明:首免和二免时间分别为21,50日龄,注射剂量分别为0.3,0.5 mL/只;各组鸡只H5和H7抗体效价产生时间和持续时间大致相同;6个品种鸡只的抗体效价在首免后14天均能够上升至4.0 lb以上,而后持续上升。50日龄之前土杂鸡、芦花鸡、瑶鸡、青花鸡、珍珠鸡和武定鸡H5最高抗体效价分别为9.7,9.7,8.4,8.6,10.6,9.5 lb, H7最高抗体效价分别为6.9,8.2,9.5,7.5,5.9,11.5 lb;50日龄后各组鸡只抗体效价开始下降,二免后抗体效价快速上升,4.0 lb以上的抗体效价维持至141日龄(除土杂鸡外),土杂鸡、芦花鸡、瑶鸡、青花鸡、珍珠鸡和武定鸡在二免后H5最高抗体效价分别为10.5,10.8,11.3,11.1,11.4,11.3 lb, H7最高抗体效价分别为9.1,10.1,9.7,9.9,8.5,10.9 lb,直至155日龄衰减到4.0 lb以下(土杂鸡在141日龄抗体效价降至4.0 lb以下)。6个云南地方品种鸡种对禽流感病毒(H5+H7)二价灭活疫苗均能产生良好的免疫应答,各组鸡只之间的H5和H7抗体消长规律并无明显差异。说明此次使用的重组禽流感病毒(H5+H7)二价灭活疫苗的免疫效果好且产生的H5和H7两种抗体能在鸡体内很好地发挥各自的免疫作用,无明显的互相干扰现象。In order to explore the immune effect of the recombinant Avian influenza virus(H5+H7) bivalent inactivated vaccine(H5 N1 Re-8 strain + H7 N9 H7-Re1 strain) launched by the Ministry of Agriculture and Rural Affairs in 2017,the experiment determined the time and injection dose for the first immunization and the second immunization based on the results of the maternal antibody test and the law of antibody growth and decline;vaccination was carried out by injection on six breeds of Yunnan local chicken: local hybrid chicken, Luhua chicken, Yao chicken, Qinghua chicken, Guinea fowl and Wuding chicken. Hemagglutination(HA) and hemagglutination inhibition(HI) tests were used to determine the Antibody titers on 9,1 days before immunization, 7,14,21,28 days after the first immunization, and 7,14,21,28,35,49,63,77,91,105 days after the second immunization;at the same time the antibody growth curve was drawn, and the avian influenza antibody levels of six breeds of Yunnan local chicken were analyzed and the immune effect were evaluated. The results showed that the time of the first immunization and the second immunization were 21 and 50 days old, respectively, and the injection dose was 0.3 and 0.5 mL/chicken, respectively;the production time and duration of H5 and H7 antibody titer of chickens in each group were roughly the same. After the first vaccination, the antibody titer of the six breeds of chickens can rise to more than 4.0 lb in 14 days after the first vaccination, and then continued to rise;before 50 days of age, the highest antibody titers of H5 among local hybrid chicken, Luhua chicken, Yao chicken, Qinghua chicken, Guinea fowl and Wuding chicken, were 9.7,9.7,8.4,8.6,10.6,9.5 lb, respectively, and the highest antibody titers of H7 were 6.9,8.2,9.5,7.5,5.9, 11.5 lb, respectively. After 50 days of age, the antibody titer of the chickens in each group began to decline, and the antibody titer increased rapidly after the second immunization, and the antibody titer of 4.0 lb or more was maintained to 141 days of age

关 键 词：禽流感 重组禽流感病毒 免疫效果 二价灭活菌 云南地方鸡 

分 类 号：S852.65[农业科学—基础兽医学] S852.4[农业科学—兽医学]