圈养非人灵长类动物肠道大肠杆菌的耐药性与整合子携带研究  

Study on antimicrobial resistance and integron carrying of Escherichia coli isolates in captive non-human primates

作　　者：朱子琦 程跃红 王利勤[3] 潘舒蕾 赵波[3] 冯丹 魏斌 李向勇 彭广能[1] 钟志军[1] ZHU Ziqi;CHENG Yuehong;WANG Liqin;PAN Shulei;ZHAO Bo;FENG Dan;WEI Bin;LI Xiangyong;PENG Guangneng;ZHONG Zhijun(College of Veterinary Medicine,Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China;Wolong National Natural Reserve Administration Bureau,Wenchuan 623006,China;The Chengdu Zoo,Institute of Wild Animals,Chengdu 610081,China;Animal Disease Prevention and Control Center of Qingxu County,Qingxu 030440,China;Changsha Ecological Zoo,Changsha 410118,China)

机构地区：[1]四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室,成都611130 [2]四川卧龙国家级自然保护区管理局,四川汶川623006 [3]成都动物园成都市野生动物研究所,成都610081 [4]山西省清徐县动物疾病预防控制中心,山西清徐030440 [5]长沙生态动物园,长沙410118

出　　处：《黑龙江畜牧兽医》2021年第13期122-129,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家重点研发计划项目(2018YFD0500900,2016YFD0501009);成都大熊猫繁育研究基金会项目(CPF2017-05,CPF2015-4);成都动物园园级课题(18-01)

摘　　要：为了了解非人灵长类肠道大肠杆菌(Escherichia coli)的耐药性以及耐药基因和整合子的携带情况,试验采用K-B纸片扩散法对56株分离自非人灵长类的大肠杆菌进行22种抗菌药物的敏感性测试,同时采用PCR方法检测菌株中耐药基因及整合子(Ⅰ、Ⅱ、Ⅲ类)携带情况。结果表明:56株大肠杆菌对22种抗菌药物呈不同程度耐药,其中对阿莫西林(87.50%)、阿莫西林/克拉维酸(82.14%)、多西环素(55.35%)和四环素(50.00%)的耐药性较高。22种耐药基因中blaTEM检出率最高(35/56,62.50%),其次为tet(A)(34/56,60.71%)和sul2(22/56,39.29%),其余耐药基因检出率为0~17.86%。56株大肠杆菌中共有9株检测出Ⅰ类整合子(9/56,16.07%),未检出Ⅱ,Ⅲ类整合子。说明此次分离的非人灵长类肠道大肠杆菌对抗菌药物呈不同程度的耐药性;耐药基因检出率高且复杂;整合子介导的耐药基因传播是菌株产生多重耐药表型的机制之一。To investigate the drug resistance of Escherichia coli in intestinal tract of non-human primates, and the carrying of drug-resistant genes and integrons, in this study, the susceptibility of 56 strains of E.coli isolated from non-human primates to 22 antimicrobial agents was tested by Kirly-Bauer disc diffusion method. At the same time, PCR method was used to detect the antibiotic resistant genes and integron(class Ⅰ, Ⅱ, Ⅲ) carrying in E.coli isolates. The results showed that 56 strains of E.coli were resistant to 22 antimicrobial agents to different degrees, among which the resistance to amoxicillin(87.50%), amoxicillin-clavic acid(82.14%), doxycycline(55.35%) and tetracycline(50.00%) were highly resistant. Among the 22 drug-resistant genes, the detection rate of blaTEM was the highest(35/56, 62.50%), followed by tet(A)(34/56, 60.71%) and sul2(22/56, 39.29%), while the detection rate of other drug-resistant genes was between 0-17.86%. A total of 9 strains(9/56, 16.07%) of the 56 strains of E.coli were found to carry class Ⅰ integron, and class Ⅱ or class Ⅲ were not detected in 56 E. coli isolates. It indicated that the non-human primate intestinal E. coli isolated in this study showed different degrees of resistance to antimicrobial agents. The detection rate of antibiotic resistant gene was high and complex. Integron-mediated transmission of drug-resistant genes was one of the mechanisms for strains to produce multiple drug-resistant phenotypes.

关 键 词：非人灵长类 大肠杆菌 耐药性 耐药基因 整合子 

分 类 号：S852.612[农业科学—基础兽医学] S865.31[农业科学—兽医学]