聚丙烯酰胺类油田添加剂生物毒性测试方法  

Test method for biological toxicity of polyacrylamide oil field additives

作　　者：马跃[1] 宫琦 李术元[1] 赖晓晴 MA Yue;GONG Qi;LI Shu-yuan;LAI Xiao-qing(School of Science, China University of Petroleum, Beijing 102249, China;Drilling Fluid Station, CNPC Engineering Technology R& D Company Limited, Beijing 102206, China)

机构地区：[1]中国石油大学理学院,北京102249 [2]中国石油集团工程技术研究院有限公司钻井液所,北京102206

出　　处：《沈阳工业大学学报》2021年第4期402-406,共5页Journal of Shenyang University of Technology

基　　金：辽宁省教育厅一般计划项目(LQGD2017020).

摘　　要：为了有效解决明亮发光细菌T3价格暴涨且供货不稳定的问题,采用费氏弧菌为测试菌种,ZnSO4·7H2O为参比毒物,通过单因素实验探讨了不同时间内费氏弧菌的稳定性,考察了不同浓度的参比毒物对菌种发光强度和抑制率的影响,研究了费氏弧菌在油田添加剂生物毒性测试中的应用.结果表明:费式弧菌在测试过程中存活性高、稳定性好;参比毒物ZnSO4·7H2O浓度越大,发光强度越低,抑制率越高;费氏弧菌测得的降滤失剂的毒性大于包被剂,最佳测试时间为15~30 min.In order to effectively solve the problem of expensive price and unstable supply of photobacterium phosphoreum T3,vibrio fischeri was used as test bacteria,ZnSO4·7H2O was used as the reference poison,and the stability of vibrio fischeri within different time periods was studied by single factor experiments.The effect of reference poisons with different concentrations on luminous intensity and inhibition rate was investigated,and the application of vibrio fischeri in the biological toxicity tests of oil field additives was studied.The results show that the vibrio fischeri has high activity and stability during the tests.The higher the concentration of reference poison ZnSO4·7H2O,the lower the luminous intensity,and the higher the inhibition rate.The toxicity of filtrate loss reducer measured by vibrio fischeri is greater than that of coating agent,and the optimal test time is within the range from 15 to 30 min.

关 键 词：聚丙烯酰胺 丙烯酰胺 油田添加剂 生物毒性 费氏弧菌 明亮发光细菌T3 抑制率 发光强度 

分 类 号：TE991[石油与天然气工程—石油机械设备]