左归丸促进K562细胞向红系分化的分子机制  

The Molecular Mechanism of Zuogui Wan(左归丸)in Promoting the Erythroid Differentiation of K562 Cells

作　　者：赵汗青 张群秀[1] 王文娟[1] ZHAO Han-qing;ZHANG Qun-xiu;WANG Wen-juan(School of Chinese Medicine,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《中医药导报》2021年第7期56-61,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(81774176);首都中医药研究专项重点项目(2016ZY02)。

摘　　要：目的:探讨左归丸改善肿瘤相关性贫血的机制。方法:培养K562细胞,以左归丸含药血清进行干预,同时设置空白血清对照、阳性药对照,采用CCK8测定细胞增殖活性,瑞氏-吉姆萨染色观察细胞形态,联苯胺法分析表达血红蛋白的阳性细胞率及血红蛋白含量,流式细胞仪检测细胞CD71表达率,Western blotting、real-time PCR分别分析GATA-1、EPO、EPOR蛋白与基因表达情况。结果:左归丸血清可明显抑制K562细胞增殖(P<0.05或P<0.01),降低细胞核浆比,促进细胞表达血红蛋白(P<0.01),增加细胞CD71表达率(P<0.05或P<0.01),上调GATA-1蛋白表达(P<0.01或P<0.05),上调GATA-1 m RNA、EPO m RNA、EPOR m RNA表达(P<0.01或P<0.05)。结论:左归丸血清可促进K562细胞向红系分化,其机制与调控GATA-1、EPO、EPOR表达密切相关。Objective:To explore the mechanism of Zuogui Wan in improving tumor-related anemia.Methods:K562 cells were cultured and intervened with serum containing Zuogui Wan.At the same time,a blank serum control and a positive drug control were set.CCK8 was used to determine cell proliferation activity.Wright-Giemsa staining was used to observe cell morphology.The positive cell rate and hemoglobin content was analyzed by Benzidine method.The expression of CD71 was detected by flow cytometry.Western blotting and real-time PCR were used to analyze the protein and gene expression of GATA-1,EPO and EPOR.Results:Zuogui Wan serum can significantly inhibit the proliferation of K562 cells(P<0.05 or P<0.01),reduce the ratio of nucleus to plasma,promote the expression of hemoglobin in cells(P<0.01),and increase the expression rate of CD71 in cells(P<0.05 or P<0.01),up-regulate the expression of GATA-1 protein(P<0.01 or P<0.05)and up-regulate the expression of GATA-1 mRNA,EPO mRNA,and EPOR mRNA(P<0.01 or P<0.05).Conclusion:Zuogui Wan serum can promote the differentiation of K562 cells into erythroid,and its mechanism is closely related to the regulation of the expression of GATA-1,EPO and EPOR.

关 键 词：肿瘤相关性贫血 左归丸 红系分化 K562细胞 

分 类 号：R285.5[医药卫生—中药学]