The WRKY transcription factor HpWRKY44 regulates CytP450-like1 expression in red pitaya fruit(Hylocereus polyrhizus)  

作　　者：Mei-nv Cheng Zi-juan Huang Qing-zhu Hua Wei Shan Jian-fei Kuang Wang-jin Lu Yong-hua Qin Jian-ye Chen 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China,Ministry of Agriculture,College of Horticulture,South China Agricultural University,Guangzhou,510642,China

出　　处：《Horticulture Research》2017年第1期192-200,共9页园艺研究（英文）

基　　金：This work was supported by the Science and Technology Planning Project of Guangdong Province(2014B020202010);Science and Technology Program of Guangzhou(201704020003 and 2014Y2-00164);YangFan Innovative and Entepreneurial Research Team Project(2014YT02H013);the earmarked fund for Guangdong Modern Agro-industry Technology Research System(2016LM112).

摘　　要：Red pitaya(Hylocereus polyrhizus)fruit is a high-value,functional food,containing a high level of betalains.Several genes potentially related to betalain biosynthesis,such as cytochrome P450-like(CytP450-like),have been identified in pitaya fruit,while their transcriptional regulation remains unclear.In this work,the potential involvement of a WRKY transcription factor,HpWRKY44,in regulating CytP450-like1 expression in pitaya fruit was examined.HpWRKY44,a member of the Group 1 WRKY family,contains two conserved WRKY motifs and is localized in the nucleus.HpWRKY44 also exhibits trans-activation ability.Gene expression analysis showed that the expression of HpCytP450-like1 and HpWRKY44 increased steadily during pitaya fruit coloration,which corresponded with the production of elevated betalain levels in the fruit.HpWRKY44 was also demonstrated to directly bind to and activate the HpCytP450-like1 promoter via the recognition of the W-box element present in the promoter.Collectively,our findings indicate that HpWRKY44 transcriptionally activates HpCytP450-like1,which perhaps,at least in part,contributes to betalain biosynthesis in pitaya fruit.The information provided in the current study provides novel insights into the regulatory network associated with betalain biosynthesis during pitaya fruit coloration.

关 键 词：ELEVATED ACTIVATION finding 

分 类 号：Q94[生物学—植物学]