Handheld endomicroscope using a fiber-optic harmonograph enables real-time and in vivo confocal imaging of living cell morphology and capillary perfusion  

作　　者：Kyungmin Hwang Yeong-Hyeon Seo Daniel YKim Jinhyo Ahn Soyoung Lee Kyung Hee Han Koun-Hee Lee Sangyong Jon Pilhan Kim Kate EYu Hyungsin Kim Shin-Hyuk Kang Ki-Hun Jeong 

机构地区：[1]Department of Bio and Brain Engineering,KAIST and KAIST Institute of Health Science and Technology,Daejeon 34141,Republic of Korea [2]Graduate School of Nanoscience and Technology,KAIST and KAIST Institute of Health Science and Technology,Daejeon 34141,Republic of Korea [3]Department of Biological Sciences,KAIST and KAIST Institute for the BioCentury,Daejeon 34141,Republic of Korea [4]Hyunjoo In-Tech,Seoul 08390,Republic of Korea [5]VPIX Medical,Inc,Deajeon 34141,Republic of Korea [6]Graduate School of Medical Science and Engineering,Daejeon 34141,Republic of Korea [7]Department of Neurosurgery,Korea University Anam Hospital,Korea University Medicine,Seoul 02842,Korea

出　　处：《Microsystems & Nanoengineering》2020年第1期449-459,共11页微系统与纳米工程（英文）

基　　金：A grant of the Korean Health Technology R&D Project,Ministry of Health&Welfare,Republic of Korea(HI13C2181);from the National Research Foundation of Korea(NRF)(2016R1A2B301306115);TIPS Program of Ministry of SMEs and Startups(S2566909).

摘　　要：Confocal laser endomicroscopy provides high potential for noninvasive and in vivo optical biopsy at the cellular level.Here,we report a fully packaged handheld confocal endomicroscopic system for real-time,high-resolution,and in vivo cellular imaging using a Lissajous scanning fiber-optic harmonograph.The endomicroscopic system features an endomicroscopic probe with a fiber-optic harmonograph,a confocal microscope unit,and an image signal processor.The fiber-optic harmonograph contains a single mode fiber coupled with a quadrupole piezoelectric tube,which resonantly scans both axes at~1 kHz to obtain a Lissajous pattern.The fiber-optic harmonograph was fully packaged into an endomicroscopic probe with an objective lens.The endomicroscopic probe was hygienically packaged for waterproofing and disinfection of medical instruments within a 2.6-mm outer diameter stainless tube capable of being inserted through the working channel of a clinical endoscope.The probe was further combined with the confocal microscope unit for indocyanine green imaging and the image signal processor for high frame rate and high density Lissajous scanning.The signal processing unit delivers driving signals for probe actuation and reconstructs confocal images using the auto phase matching process of Lissajous fiber scanners.The confocal endomicroscopic system was used to successfully obtain human in vitro fluorescent images and real-time ex vivo and in vivo fluorescent images of the living cell morphology and capillary perfusion inside a single mouse.

关 键 词：fiber PROCESSOR CONFOCAL 

分 类 号：TH773[机械工程—仪器科学与技术]