Nanovolcano microelectrode arrays: toward long- term on-demand registration of transmembrane action potentials by controlled electroporation  

作　　者：Benoit X.E.Desbiolles Etienne de Coulon Nicolas Maino Arnaud Bertsch Stephan Rohr Philippe Renaud 

机构地区：[1]Laboratory of Microsystems LMIS4,Ecole Polytechnique Federale de Lausanne,Lausanne,Switzerland [2]Department of Physiology,Laboratory of Cellular Optics II,University of Bern,Bern,Switzerland

出　　处：《Microsystems & Nanoengineering》2020年第1期578-589,共12页微系统与纳米工程（英文）

基　　金：This work was partially funded by the Swiss National Science Foundation(grant number 200021_175943 to P.R.and 310030_169234 to S.R.)。

摘　　要：Volcano-shaped microelectrodes(nanovolcanoes)functionalized with nanopatterned self-assembled monolayers have recently been demonstrated to report cardiomyocyte action potentials after gaining spontaneous intracellular access.These nanovolcanoes exhibit recording characteristics similar to those of state-of-the-art micro-nanoelectrode arrays that use electroporation as an insertion mechanism.In this study,we investigated whether the use of electroporation improves the performance of nanovolcano arrays in terms of action potential amplitudes,recording durations,and yield.Experiments with neonatal rat cardiomyocyte monolayers grown on nanovolcano arrays demonstrated that electroporation pulses with characteristics derived from analytical models increased the efficiency of nanovolcano recordings,as they enabled multiple on-demand registration of intracellular action potentials with amplitudes as high as 62 mV and parallel recordings in up to~76%of the available channels.The performance of nanovolcanoes showed no dependence on the presence of functionalized nanopatterns,indicating that the tip geometry itself is instrumental for establishing a tight seal at the cell–electrode interface,which ultimately determines the quality of recordings.Importantly,the use of electroporation permitted the recording of attenuated cardiomyocyte action potentials during consecutive days at identical sites,indicating that nanovolcano recordings are nondestructive and permit long-term on-demand recordings from excitable cardiac tissues.Apart from demonstrating that less complex manufacturing processes can be used for next-generation nanovolcano arrays,the finding that the devices are suitable for performing on-demand recordings of electrical activity from multiple sites of excitable cardiac tissues over extended periods of time opens the possibility of using the devices not only in basic research but also in the context of comprehensive drug testing.

关 键 词：arrays RECORDING action 

分 类 号：TH77[机械工程—仪器科学与技术] R318[机械工程—精密仪器及机械]