On-chip integrated optical stretching and electrorotation enabling single-cell biophysical analysis  

作　　者：Liang Huang Fei Liang Yongxiang Feng Peng Zhao Wenhui Wang 

机构地区：[1]Department of Precision Instrument,State Key Laboratory of Precision Measurement Technology and Instrument,Tsinghua University,Beijing,China [2]School of Instrument Science and Opto-Electronics Engineering,Hefei University of Technology,Hefei,China

出　　处：《Microsystems & Nanoengineering》2020年第1期880-893,共14页微系统与纳米工程（英文）

基　　金：This work was supported by the NSFC(nos.61774095 and 21727813);the National Key R&D Program(no.2016YFC0900200);the One-Thousand Young Talent Program of China.

摘　　要：Cells have different intrinsic markers such as mechanical and electrical properties,which may be used as specific characteristics.Here,we present a microfluidic chip configured with two opposing optical fibers and four 3D electrodes for multiphysical parameter measurement.The chip leverages optical fibers to capture and stretch a single cell and uses 3D electrodes to achieve rotation of the single cell.According to the stretching deformation and rotation spectrum,the mechanical and dielectric properties can be extracted.We provided proof of concept by testing five types of cells(HeLa,A549,HepaRG,MCF7 and MCF10A)and determined five biophysical parameters,namely,shear modulus,steady-state viscosity,and relaxation time from the stretching deformation and area-specific membrane capacitance and cytoplasm conductivity from the rotation spectra.We showed the potential of the chip in cancer research by observing subtle changes in the cellular properties of transforming growth factor beta 1(TGF-β1)-induced epithelial–mesenchymal transition(EMT)A549 cells.The new chip provides a microfluidic platform capable of multiparameter characterization of single cells,which can play an important role in the field of single-cell research.

关 键 词：ROTATION STRETCHING fibers 

分 类 号：TN25[电子电信—物理电子学]