The GAMYB-like gene SlMYB33 mediates flowering and pollen development in tomato  

作　　者：Yan Zhang Bo Zhang Tongwen Yang Jie Zhang Bin Liu Xiangqiang Zhan Yan Liang 

机构地区：[1]College of Horticulture,Northwest A&F University,Yangling 712100 Shaanxi,P.R.China [2]School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Horticulture Research》2020年第1期903-918,共16页园艺研究（英文）

基　　金：supported by the National Key Research and Development Program of China(2016YFD0101703 and 2016YFD0100204-30);Basic Research Projects of Natural Science of Shaanxi Province(2018JQ3063);National Natural Science Foundation of China(31671273).

摘　　要：GAMYBs are positive GA signaling factors that exhibit essential functions in reproductive development,particularly in anther and pollen development.However,there is no direct evidence of the regulation of any GAMYB in these biological processes in tomato(Solanum lycopersicum).Here,we identified a tomato GAMYB-like gene,SlMYB33,and characterized its specific roles.SlMYB33 is predominately expressed in the stamens and pistils.During flower development,high mRNA abundance of SlMYB33 is detected in both male and female organs,such as microspore mother cells,anthers,pollen grains,and ovules.Silencing of SlMYB33 leads to delayed flowering,aberrant pollen viability,and poor fertility in tomato.Histological analyses indicate that SlMYB33 exerts its function in pollen development in the mature stage.Further transcriptomic analyses imply that the knockdown of SlMYB33 significantly inhibits the expression of genes related to flowering in shoot apices,and alters the transcription of genes controlling sugar metabolism in anthers.Taken together,our study suggests that SlMYB33 regulates tomato flowering and pollen maturity,probably by modulating the expression of genes responsible for flowering and sugar metabolism,respectively.

关 键 词：METABOLISM SUGAR MEDIA 

分 类 号：S641.2[农业科学—蔬菜学]