MYB41,MYB107,and MYC2 promote ABA-mediated primary fatty alcohol accumulation via activation of AchnFAR in wound suberization in kiwifruit  

作　　者：Xiaopeng Wei Linchun Mao Xiaobo Wei Ming Xia Changjie Xu 

机构地区：[1]College of Biosystems Engineering and Food Science,Zhejiang Key Laboratory of Agro-Food Processing,Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs,Zhejiang University,310058 Hangzhou,China [2]Ningbo Research Institute,Zhejiang University,315100 Ningbo,China [3]Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,310058 Hangzhou,China

出　　处：《Horticulture Research》2020年第1期1666-1675,共10页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.31772365 and 31972468);the National Key Research and Development Program of China(No.2018YFD0401303).

摘　　要：Wound damage triggers the accumulation of abscisic acid(ABA),which induces the expression of a large number of genes involved in wound suberization in plants.Fatty acyl-CoA reductase(FAR)catalyzes the generation of primary fatty alcohols by the reduction of fatty acids in suberin biosynthesis.However,the regulatory effects of transcription factors(TFs)on AchnFAR in response to ABA are unexplored.In this study,kiwifruit AchnFAR displayed a biological function analogous to that of FAR in transiently overexpressed tobacco(Nicotiana benthamiana)leaves.The positive role of TFs,including AchnMYB41,AchnMYB107,and AchnMYC2,in the regulation of AchnFAR was identified.The three TFs could individually bind to the AchnFAR promoter to activate gene transcription in yeast one-hybrid and dualluciferase assays.Transient overexpression of TFs in tobacco leaves resulted in the upregulation of aliphatic synthesis genes(including FAR)and the increase in aliphatics,including primary alcohols,α,ω-diacids,ω-hydroxyacids,and fatty acids.Moreover,exogenous ABA treatment elevated TF-mediated AchnFAR expression and the accumulation of primary alcohols.Conversely,fluridone,an inhibitor of ABA biosynthesis,suppressed the expression of AchnFAR and TF genes and reduced the formation of primary alcohols.The results indicate that AchnMYB41,AchnMYB107,and AchnMYC2 activate AchnFAR transcription to promote ABA-mediated primary alcohol formation in wound suberization in kiwifruit.

关 键 词：WOUND mediated ELEVATED 

分 类 号：S663.4[农业科学—果树学]