The persimmon(Diospyros oleifera Cheng)genome provides new insights into the inheritance of astringency and ancestral evolution  

作　　者：Qing-gang Zhu Yang Xu Yong Yang Chang-fei Guan Qiu-yun Zhang Jing-wen Huang Don Grierson Kun-song Chen Bang-chu Gong Xue-ren Yin 

机构地区：[1]Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Zijingang Campus,Hangzhou 310058,PR China [2]State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Zijingang Campus,Hangzhou 310058,PR China [3]Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,PR China [4]College of Horticulture,Northwest A&F University,Yangling,PR China [5]Plant&Crop Sciences Division,School of Biosciences,University of Nottingham,Sutton Bonington Campus,Loughborough,UK

出　　处：《Horticulture Research》2019年第1期3-17,共15页园艺研究（英文）

基　　金：supported by the National Key Research and Development Program(2016YFD0400102);the National Natural Science Foundation of China(31672204,31722042);the Fok Ying Tung Education Foundation,China(161028),Key Agricultural New Varieties Breeding Projects funded by the Zhejiang Province Science and Technology Department(2016C02052-10);Fundamental Research Funds of CAF(CAFYBB2017SY015).

摘　　要：Persimmon(Diospyros kaki)is an oriental perennial woody fruit tree whose popular fruit is produced and consumed worldwide.The persimmon fruit is unique because of the hyperaccumulation of proanthocyanidins during fruit development,causing the mature fruit of most cultivars to have an astringent taste.In this study,we obtained a chromosome-scale genome assembly for‘Youshi’(Diospyros oleifera,2n=2x=30),the diploid species of persimmon,by integrating Illumina sequencing,single-molecule real-time sequencing,and high-throughput chromosome conformation capture techniques.The assembled D.oleifera genome consisted of 849.53 Mb,94.14%(799.71 Mb)of which was assigned to 15 pseudochromosomes,and is the first assembled genome for any member of the Ebenaceae.Comparative genomic analysis revealed that the D.oleifera genome underwent an ancientγwhole-genome duplication event.We studied the potential genetic basis for astringency development(proanthocyanidin biosynthesis)and removal(proanthocyanidin insolublization).Proanthocyanidin biosynthesis genes were mainly distributed on chromosome 1,and the clustering of these genes is responsible for the genetic stability of astringency heredity.Genome-based RNA-seq identified deastringency genes,and promoter analysis showed that most of their promoters contained large numbers of low oxygen-responsive motifs,which is consistent with the efficient industrial application of high CO2 treatment to remove astringency.Using the D.oleifera genome as the reference,SLAF-seq indicated that‘Youshi’is one of the ancestors of the cultivated persimmon(2n=6x=90).Our study provides significant insights into the genetic basis of persimmon evolution and the development and removal astringency,and it will facilitate the improvement of the breeding of persimmon fruit.

关 键 词：breeding CULTIVAR STRING 

分 类 号：S66[农业科学—果树学]