Auxin regulation involved in gynoecium morphogenesis of papaya flowers  

作　　者：Ping Zhou Mahpara Fatima Xinyi Ma Juan Liu Ray Ming 

机构地区：[1]College of Life Sciences,FAFU and UIUC Joint Center for Genomics and Biotechnology,Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,Fujian Agriculture and Forestry University,Fuzhou 350002 Fujian,China [2]Fruit Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013 Fujian,China [3]College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002 Fujian,China [4]Department of Plant Biology,University of Illinois at Urbana-Champaign,Urbana,IL 61801,USA

出　　处：《Horticulture Research》2019年第1期237-247,共11页园艺研究（英文）

基　　金：supported by startup fund from Fujian Agriculture and Forestry University and the NSF Plant Genome Research Program Award 1546890.It was also supported by National Natural Science Foundation of China(31701889);Natural Science Foundation of Fujian Province of China(2018J01601).

摘　　要：The morphogenesis of gynoecium is crucial for propagation and productivity of fruit crops.For trioecious papaya(Carica papaya),highly differentiated morphology of gynoecium in flowers of different sex types is controlled by gene networks and influenced by environmental factors,but the regulatory mechanism in gynoecium morphogenesis is unclear.Gynodioecious and dioecious papaya varieties were used for analysis of differentially expressed genes followed by experiments using auxin and an auxin transporter inhibitor.We first compared differential gene expression in functional and rudimentary gynoecium at early stage of their development and detected significant difference in phytohormone modulating and transduction processes,particularly auxin.Enhanced auxin signal transduction in rudimentary gynoecium was observed.To determine the role auxin plays in the papaya gynoecium,auxin transport inhibitor(N-1-Naphthylphthalamic acid,NPA)and synthetic auxin analogs with different concentrations gradient were sprayed to the trunk apex of male and female plants of dioecious papaya.Weakening of auxin transport by 10 mg/L NPA treatment resulted in female fertility restoration in male flowers,while female flowers did not show changes.NPA treatment with higher concentration(30 and 50 mg/L)caused deformed flowers in both male and female plants.We hypothesize that the occurrence of rudimentary gynoecium patterning might associate with auxin homeostasis alteration.Proper auxin concentration and auxin homeostasis might be crucial for functional gynoecium morphogenesis in papaya flowers.These results will lead to further investigation on the auxin homeostasis and gynoecium morphogenesis in papaya.

关 键 词：PAPAYA MORPHOGENESIS FLOWERS 

分 类 号：Q94[生物学—植物学]