Comparative proteomic analysis of cucumber powdery mildew resistance between a singlesegment substitution line and its recurrent parent  

作　　者：Xuewen Xu Xueli Liu Yali Yan Wei Wang Kiros Gebretsadik Xiaohua Qi Qiang Xu Xuehao Chen 

机构地区：[1]School of Horticulture and Plant Protection,Yangzhou University,225009 Yangzhou,Jiangsu,China

出　　处：《Horticulture Research》2019年第1期278-290,共13页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(grant nos.31672176 and 31171978);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(18KJB210014).

摘　　要：Powdery mildew(PM)is considered a major cause of yield losses and reduced quality in cucumber worldwide,but the molecular basis of PM resistance remains poorly understood.A segment substitution line,namely,SSL508-28,was developed with dominant PM resistance in the genetic background of PM-susceptible cucumber inbred line D8.The substituted segment contains 860 genes.An iTRAQ-based comparative proteomic technology was used to map the proteomes of PM-inoculated and untreated(control)D8 and SSL508-28.The number of differentially regulated proteins(DRPs)in SSL508-28 was almost three times higher than that in D8.Fourteen DRPs were located in the substituted segment interval.Comparative gene expression analysis revealed that nodulin-related protein 1(NRP1)may be a good candidate for PM resistance.Gene Ontology enrichment analysis showed that DRPs functioning in tetrapyrrole biosynthetic process,sulfur metabolic process and cell redox homeostasis were specifically enriched in the resistant line SSL508-28.DRPs categorized in the KEGG term photosynthesis increased in both lines upon PM infection,suggesting that the strategies used by cucumber may be different from those used by other crops to react to PM attacks at the initial stage.The measurement of hydrogen peroxide and superoxide anion production and net photosynthetic rate were consistent with the changes in protein abundance,suggesting that the proteomic results were reliable.There was a poor correlation between DRPs measured by iTRAQ and the corresponding gene expression changes measured by RNA-seq with the same experimental design.Taken together,these findings improve the understanding of the molecular mechanisms underlying the response of cucumber to PM infection.

关 键 词：MILDEW CROPS RESISTANCE 

分 类 号：S64[农业科学—蔬菜学]