A putative bHLH transcription factor is a candidate gene for male sterile 32,a locus affecting pollen and tapetum development in tomato  

作　　者：Xiaoyan Liu Mengxia Yang Xiaolin Liu Kai Wei Xue Cao Xiaotian Wang Xiaoxuan Wang Yanmei Guo Yongchen Du Junming Li Lei Liu Jinshuai Shu Yong Qin Zejun Huang 

机构地区：[1]College of Forestry and Horticulture,Xinjiang Agricultural University,830052 Urumqi,China [2]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,100086 Beijing,China

出　　处：《Horticulture Research》2019年第1期651-661,共11页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(No.31471889);the National Key Research and Development Program of China(No.2016YFD0101703);the Central Publicinterest Scientific Institution Basal Research Fund(No.Y2017PT52);the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(No.CAAS-ASTIP-IVFCAAS).

摘　　要：The tomato(Solanum lycopersicum)male sterile 32(ms32)mutant has been used in hybrid seed breeding programs largely because it produces no pollen and has exserted stigmas.In this study,histological examination of anthers revealed dysfunctional pollen and tapetum development in the ms32 mutant.The ms32 locus was fine mapped to a 28.5 kb interval that encoded four putative genes.Solyc01g081100,a homolog of Arabidopsis bHLH10/89/90 and rice EAT1,was proposed to be the candidate gene of MS32 because it contained a single nucleotide polymorphism(SNP)that led to the formation of a premature stop codon.A codominant derived cleaved amplified polymorphic sequence(dCAPS)marker,MS32D,was developed based on the SNP.Real-time quantitative reverse-transcription PCR showed that most of the genes,which were proposed to be involved in pollen and tapetum development in tomato,were downregulated in the ms32 mutant.These findings may aid in marker-assisted selection of ms32 in hybrid breeding programs and facilitate studies on the regulatory mechanisms of pollen and tapetum development in tomato.

关 键 词：STERILE breeding BHLH 

分 类 号：Q94[生物学—植物学]