Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks  

作　　者：Kambulu Lukusa John Kabuba 

机构地区：[1]Department of Animal and Wildlife Sciences,University of Pretoria,South Africa [2]Department of Chemical Engineering,Vaal University of Technology,South Africa

出　　处：《Asian pacific Journal of Reproduction》2021年第4期187-194,共8页亚太生殖杂志（英文版）

摘　　要：Objective:To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks.Methods:Sixteen healthy bucks were divided into two equal groups(n=8 each).The treatment group received selenium at 10-day intervals for three months.Sperm kinematic parameters,morphological parameters,mitochondrial membrane potential,plasma membrane functionality,and sperm DNA integrity were evaluated weekly pre and post-cryopreservation.Results:The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method(P<0.05).Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation(P<0.05).Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups(P<0.05).The sperm kinematic parameters such as curvilinear velocity,average path velocity,and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks(P<0.05).The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups(P<0.05).In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups,respectively(P<0.05).Conclusions:Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.

关 键 词：Post thaw CRYOPRESERVATION Saanen buck Selenium supplementation Semen collection method Sperm kinematic parameters Sperm motility Sperm quality 

分 类 号：R69[医药卫生—泌尿科学]