Genomic variants of genes associated with three horticultural traits in apple revealed by genome re-sequencing  

作　　者：Shijie Zhang Weiping Chen Lu Xin Zhihong Gao Yingjun Hou Xinyi Yu Zhen Zhang Shenchun Qu 

机构地区：[1]College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China [2]College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Horticulture Research》2014年第1期55-68,共14页园艺研究（英文）

基　　金：the Jiangsu Province Science Technology Independent Innovation Fund(CX(13)3006);the Sanxin Project of Jiangsu Province(SXGC(2013)046)for providing financial support;We also acknowledge the National Natural Science Foundation of China(31171935)for providing financial support for a portion of this study.

摘　　要：The apple(Malus×domestica Borkh.)cultivar‘Su Shuai’exhibits greater disease resistance,shorter internodes and lighter fruit flavor compared with its parents‘Golden Delicious’and‘Indo’.To obtain a comprehensive overview of the sequence variation in these three horticultural traits,the genomes of‘Su Shuai’and‘Indo’were resequenced using next-generation sequencing and compared to the genome of‘Golden Delicious’.A wide range of genetic variations were detected,including 2454406 and 18749349 single nucleotide polymorphism(SNP)and 59547 and 50143 structural variants(SVs)in the‘Indo’and‘Su Shuai’genomes,respectively.Among the SVs in‘Su Shuai’,17 genes related to disease resistance,10 genes related to Gibberellin(GA)and 19 genes associated with fruit flavor were identified.The expression patterns of eight of the SV genes were examined using reverse transcription-quantitative polymerase chain reaction(RT-qPCR).The results of this study illustrate the genomic variation in these cultivars and provide evidence for a genetic basis for the horticultural traits of disease resistance,short internodes and lighter flavor exhibited in these cultivars.These results provide a genetic basis for the phenotypic characteristics of‘Su Shuai’and,as such,these SVs could serve as gene-specific molecular markers in maker-assisted breeding of apples.

关 键 词：CULTIVAR breeding TRAITS 

分 类 号：S51[农业科学—作物学]