QTL analysis of soft scald in two apple populations  

作　　者：Kendra A McClure Kyle M Gardner Peter MA Toivonen Cheryl R Hampson Jun Song Charles F Forney John DeLong Istvan Rajcan Sean Myles 

机构地区：[1]Department of Plant and Animal Sciences,Faculty of Agriculture,Dalhousie University,Truro,B2N 5E3,Nova Scotia,Canada [2]Department of Plant Agriculture,Crop Science Building,University of Guelph,Guelph,N1G 2W1,Ontario,Canada [3]Agriculture and Agri-Food Canada,Fredericton Research and Development Centre,Fredericton,E3B 4Z7,New Brunswick,Canada [4]Agriculture and Agri-Food Canada,Summerland Research and Development Centre,Summerland,V0H 1Z0,British Columbia,Canada [5]Agriculture and Agri-Food Canada,Kentville Research and Development Centre,Kentville,B4N 1J5,Nova Scotia,Canada

出　　处：《Horticulture Research》2016年第1期117-123,共7页园艺研究（英文）

基　　金：This project was funded by the A-Base research(NOI-1238)of Agriculture and Agri-Food Canada;This research was also supported in part by funding from the Canada Research Chairs program(SM)and the National Sciences and Engineering Research Council of Canada(SM,KM).

摘　　要：The apple(Malus×domestica Borkh.)is one of the world’s most widely grown and valuable fruit crops.With demand for apples year round,storability has emerged as an important consideration for apple breeding programs.Soft scald is a cold storage-related disorder that results in sunken,darkened tissue on the fruit surface.Apple breeders are keen to generate new cultivars that do not suffer from soft scald and can thus be marketed year round.Traditional breeding approaches are protracted and labor intensive,and therefore marker-assisted selection(MAS)is a valuable tool for breeders.To advance MAS for storage disorders in apple,we used genotyping-by-sequencing(GBS)to generate high-density genetic maps in two F1 apple populations,which were then used for quantitative trait locus(QTL)mapping of soft scald.In total,900 million DNA sequence reads were generated,but after several data filtering steps,only 2%of reads were ultimately used to create two genetic maps that included 1918 and 2818 single-nucleotide polymorphisms.Two QTL associated with soft scald were identified in one of the bi-parental populations originating from parent 11W-12-11,an advanced breeding line.This study demonstrates the utility of next-generation DNA sequencing technologies for QTL mapping in F1 populations,and provides a basis for the advancement of MAS to improve storability of apples.

关 键 词：BREEDING CULTIVAR APPLE 

分 类 号：S51[农业科学—作物学]