Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine  

作　　者：Stefano Pessina Luisa Lenzi Michele Perazzolli Manuela Campa Lorenza Dalla Costa Simona Urso Giampiero Valè Francesco Salamini Riccardo Velasco Mickael Malnoy 

机构地区：[1]Research and Innovation Centre,Fondazione Edmund Mach,via Edmund Mach 1,San Michele all’Adige,38010,Italy [2]Wageningen UR Plant Breeding,Wageningen University and Research Centre,PO Box 386,Wageningen,6700,AJ,The Netherlands [3]Department of Ecological and Biological Sciences,Universitàdella Tuscia,Via San Camillo de Lellis,Viterbo,01100,Italy [4]Council for Agricultural Research and Economics(CREA),Rice Research Unit,S.S.11 per Torino km 25,Vercelli,13100,Italy [5]Council for Agricultural Research and Economics(CREA),Genomics Research Centre,Via San Protaso,302,Fiorenzuola d’Arda,29017,Piacenza,Italy

出　　处：《Horticulture Research》2016年第1期248-256,共9页园艺研究（英文）

基　　金：This work was funded by the autonomous province of Trento(Italy)and the GMPF PhD program.

摘　　要：Erysiphe necator is the causal agent of powdery mildew(PM),one of the most destructive diseases of grapevine.PM is controlled by sulfur-based and synthetic fungicides,which every year are dispersed into the environment.This is why PM-resistant varieties should become a priority for sustainable grapevine and wine production.PM resistance can be achieved in other crops by knocking out susceptibility S-genes,such as those residing at genetic loci known as MLO(Mildew Locus O).All MLO S-genes of dicots belong to the phylogenetic clade V,including grapevine genes VvMLO7,11 and 13,which are upregulated during PM infection,and VvMLO6,which is not upregulated.Before adopting a gene-editing approach to knockout candidate S-genes,the evidence that loss of function of MLO genes can reduce PM susceptibility is necessary.This paper reports the knockdown through RNA interference of VvMLO6,7,11 and 13.The knockdown of VvMLO6,11 and 13 did not decrease PM severity,whereas the knockdown of VvMLO7 in combination with VvMLO6 and VvMLO11 reduced PM severity up to 77%.The knockdown of VvMLO7 and VvMLO6 seemed to be important for PM resistance,whereas a role for VvMLO11 does not seem likely.Cell wall appositions(papillae)were present in both resistant and susceptible lines in response to PM attack.Thirteen genes involved in defense were less upregulated in infected mlo plants,highlighting the early mlo-dependent disruption of PM invasion.

关 键 词：MLO CROPS MILDEW 

分 类 号：S51[农业科学—作物学]