Increased activity of MdFRK2, a high-affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves  

作　　者：Jingjing Yang Lingcheng Zhu Weifang Cui Chen Zhang Dongxia Li Baiquan Ma Lailiang Cheng Yong-Ling Ruan Fengwang Ma Mingjun Li 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,Yangling,Shaanxi,712100,China [2]Section of Horticulture,School of Integrative Plant Science,Cornell University,Ithaca,NY,14853,USA [3]School of Environmental and Life Sciences,The University of Newcastle,Callaghan,NSW,2308,Australia

出　　处：《Horticulture Research》2018年第1期54-65,共12页园艺研究（英文）

基　　金：This work was supported by the Program for the National Natural Science Foundation of China(No.31372038);by the earmarked fund for the China Agriculture Research System(CARS-28).

摘　　要：To investigate the functions of fructokinase(FRK)in apple(Malus domestica)carbohydrate metabolism,we cloned the coding sequences of MdFRK1 and MdFRK2 from the‘Royal Gala’apple.The results showed that MdFRK2 expression was extremely high in shoot tips and young fruit.Analyses of heterologously expressed proteins revealed that MdFRK2 had a higher affinity for fructose than did MdFRK1,with Km values of 0.1 and 0.62 mM for MdFRK2 and MdFRK1,respectively.The two proteins,however,exhibited similar Vmax values when their activities were significantly inhibited by high concentrations of fructose.MdFRK2 ectopic expression was associated with a general decrease in fructose concentration in transgenic lines.In leaves,increased FRK activity similarly resulted in reduced concentrations of glucose and sucrose but no alterations in sorbitol concentration.When compared with those in the untransformed control,genes involved in sorbitol synthesis(A6PR)and the degradation pathway(SDH1/2)were significantly upregulated in transgenic lines,whereas those involved in sucrose synthesis(SPS1)and other degradation processes(SUSY4,NINV1/2,and HxK2)were downregulated.The activity of enzymes participating in carbohydrate metabolism was proportional to the level of gene expression.However,the growth performance and photosynthetic efficiency did not differ between the transgenic and wild-type plants.These results provide new genetic evidence to support the view that FRK plays roles in regulating sugar and sorbitol metabolism in Rosaceae plants.

关 键 词：synthesis transformed sugar 

分 类 号：O62[理学—有机化学]