Characterization of increased cuticular wax mutant and analysis of genes involved in wax biosynthesis in Dianthus spiculifolius  

作　　者：Aimin Zhou Enhui Liu Jiao Liu Shuang Feng Shufang Gong Jingang Wang 

机构地区：[1]College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,150030,China [2]Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field(SAVER),Ministry of Education,Alkali Soil Natural Environmental Science Center(ASNESC),Northeast Forestry University,Harbin,150040,China

出　　处：《Horticulture Research》2018年第1期384-392,共9页园艺研究（英文）

基　　金：This work was supported by grants from the Natural Science Foundation of Heilongjiang Province of China,no.C2016024;the China Postdoctoral Science Foundation,no.2016M601409;the Heilongjiang Postdoctoral Fund to Pursue Scientific Research in Heilongjiang Province of China,no.LBH-Z16024;the Young Talent Project of Northeast Agricultural University of China,no.16QC06.

摘　　要：Cuticular wax formation on the surface of plant leaves is associated with drought-stress tolerance.The identification of wax biosynthesis-related genes will contribute to the genetic improvement of drought resistance in plants.In this study,we characterize a novel Dianthus spiculifolius mutant with increased cuticular wax.The mutant exhibited stronger drought resistance as indicated by less leaf wilting and death,higher leaf relative water content and water retention capacity,and slower water loss and chlorophyll extraction than did the wild type during drought treatment.In the mutant leaves,2730 upregulated and 2151 downregulated differentially expressed genes(DEGs)were identified by transcriptome sequencing.A wax biosynthesis pathway of the identified DEGs was significantly enriched.Finally,three key genes(DsCER1,DsMAH1,and DsWSD1)involved in wax biosynthesis were identified and verified by qPCR.These results suggest that differential expression of DEGs involved in wax biosynthesis may be associated with the increase in cuticular wax in the mutant.Taken together,our results help elucidate wax formation patterns in D.spiculifolius.Furthermore,the DEGs involved in wax biosynthesis identified here may be valuable genetic resources for improving plant stress tolerance through increased accumulation of cuticular wax.

关 键 词：DROUGHT SLOWER ANALYSIS 

分 类 号：S51[农业科学—作物学]