A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function  

作　　者：Man Zhu Jiajia Lin Junli Ye Rui Wang Chao Yang Jinli Gong Yun Liu Chongling Deng Ping Liu Chuanwu Chen Yunjiang Cheng Xiuxin Deng Yunliu Zeng 

机构地区：[1]Key Laboratory of Horticultural Plant Biology(Ministry of Education),College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan,430070,China [2]Institute of Citrus Science,Huazhong Agricultural University,Wuhan,430070,China [3]Shanghai Applied Protein Technology Co.Ltd,Shanghai,200233,China [4]Guangxi Citrus Research Institute,Guangxi,541004,China

出　　处：《Horticulture Research》2018年第1期879-889,共11页园艺研究（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(NSFC,grant nos.31501739);the National Basic Research Program of China(973 project No.2013CB127105);Huazhong Agricultural University Scientific&Technological Self-innovation Foundation(No.2662015BQ034).

摘　　要：Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit.

关 键 词：CITRUS COMPREHENSIVE METABOLISM 

分 类 号：S66[农业科学—果树学]