生长素IAA诱导红萍结孢的研究  

Auxin IAA-Induced Azolla Sporulation

作　　者：徐国忠[1,2] 郑向丽 王俊宏[1,2] 邓素芳 杨有泉[1,2] 应朝阳 XU Guozhong;ZHENG Xiangli;WANG Junhong;DENG Sufang;YANG Youquan;YING Zhaoyang(Agricultural Ecology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;National Azolla Germplasm Resource Center,Fuzhou,Fujian 350013,China)

机构地区：[1]福建省农业科学院农业生态研究所,福建福州350013 [2]国家红萍种质资源中心,福建福州350013

出　　处：《福建农业学报》2021年第6期713-718,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划公益类专项(2018R1010-2);现代农业产业技术体系建设专项(CARS-22)。

摘　　要：【目的】探索生长素IAA诱导红萍结孢的可行性。【方法】在网室自然条件下进行,采用质量浓度为0(对照)、1、5、10、15、20μg·mL^(-1)的生长素(IAA)对生长期的红萍1001、3001和4018进行不同浓度喷施处理,每周喷施一次,至结孢出现停止喷施。止喷施。【结果】IAA处理5个月后,1、5和10μg·mL^(-1)的细绿萍1001开始结孢,质量浓度为5μg·mL^(-1)时,结孢率、孢子果数量、孢子果䧳雄比均优于其他处理及对照。在5μg·mL^(-1)时,结孢率为15.0%、孢子果数量为6.31个、孢子果䧳雄比为0.62∶1;在10μg·mL^(-1)时,结孢率为6.3%、孢子果数量为4.12个、孢子果䧳雄比为0.53∶1;在1μg·mL^(-1)时,结孢率为4.7%、孢子果数量为3.90个、孢子果䧳雄比为0.51∶1。其他品种、处理都未能诱导出孢子果。细绿萍1001经IAA处理后,在1、5和10μg·mL^(-1)时萍体的大小和C/N比有明显提高,其余处理和对照无显著差异;卡州萍3001和小叶萍4018处理萍体大小和C/N比均无显著差异。【结论】生长素IAA能够诱导细绿萍1001结孢,其中IAA质量浓度为5μg·mL^(-1)时诱导效果最佳,可在生产上进行应用。【Objective】Auxin IAA-induced sporulation of Azolla was investigated.【Method】Under natural conditions in a netted chamber,auxin IAA solutions in concentration of 0(control),1,5,10,15,or 20μg·mL^(-1)was sprayed once-a-week on Azolla filiculoides Lamarck 1001,A.caroliniana wild 3001,and A.microphylla Kaulfus 4018 during vegetative growth period for the duration until sporocarps appeared.【Result】After five months of spraying with 1,5,or 10μg·mL^(-1)IAA solution,sporocarps began to form only on Azolla 1001.The sporulation rate,spore count,and female/male ratio were 15.0%,6.31,and 0.62∶1,respectively,under the 5μg·mL^(-1)application,which significantly differed from 6.3%,4.12,and 0.53∶1,respectively,under the 10μg·mL^(-1)application,4.7%,3.90,and 0.51∶1,respectively,under the 1μg·mL^(-1)application,and control.In addition,the size and C/N ratio of Azolla 1001 significantly increased by the spraying,as compared to control,whereas none observed on the other two ferns.【Conclusion】Auxin IAA could induce the sporulation of A.filiculoides Lamarck 1001.Spraying IAA at the concentration of 5μg·mL^(-1)on the ferns appeared feasible for field application.

关 键 词：激素 生长素 红萍 结孢 诱导 

分 类 号：S555.1[农业科学—作物学]