木醋液对龙芽楤木茎段水培抑菌效果的研究  

Study on the Antibacterial Effect of Wood Vinegar on Stem Hydroponics of Aralia elata (Miq)Seem

作　　者：申健[1] 刘德江[1] 李淑贤[2] 丛慧颖[1] 田立娟[1] SHEN Jian;LIU De-jiang;LI Shu-xian(Life Sciences College,Jiamusi University,Jiamusi,Heilongjiang 154007;Pharmacy College,Jiamusi University,Jiamusi,Heilongjiang 154007)

机构地区：[1]佳木斯大学生命科学学院,黑龙江佳木斯154007 [2]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2021年第15期51-54,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省教育厅基础研究项目(2016-KYYWF-0576);佳木斯大学博士基金项目(22Zb201512)。

摘　　要：为了控制龙芽楤木茎段水培中污染问题,采用不同稀释倍数木醋液进行培养。通过抑菌圈和细菌、真菌数量检测研究木醋液的抑菌效果。通过嫩芽生长和营养成分含量测定筛选出较适宜木醋液浓度。结果表明,木醋液在抑制细菌和真菌方面具有一定的作用,且随着木醋液稀释倍数的增大抑菌作用逐渐降低。木醋原液处理细菌抑菌圈直径为28.05 mm,真菌抑菌圈直径为17.62 mm。木醋液各处理细菌和真菌增长速度减缓,低于对照增长速度。稀释30倍处理,嫩芽生长及营养成分含量表现较好。嫩芽长度为15.9 cm,鲜重为27.58 g,叶绿素含量为0.436 mg/g,蛋白质含量为8.9 g/kg,可溶性总糖含量为23.86 g/kg,维生素C含量为187.3 mg/kg。生产实践中,综合考虑抑菌活性及嫩芽生长等情况,可以选择稀释30倍木醋液,10 d更换一次培养液进行培养。In order to control the contamination of stem in the hydroponics,different dilution ratios of wood vinegar were used for culture.The antibacterial effect of wood vinegar was studied by measuring inhibitory zone diameter and the number of bacteria and fungi.The optimum wood vinegar concentration was determined by the growth of buds and the content of nutrients.The results showed that wood vinegar had certain inhibitory effect on bacteria and fungi,and the inhibitory effect gradually decreased with the increase of dilution ratio of wood vinegar.The inhibitory zone diameter of bacteria of wood vinegar was 28.05 mm,and that of fungi was 17.62 mm.The growth rate of bacteria and fungi in wood vinegar was slower than that in control.The growth and nutrient content of the buds were better under 30 times dilution.The length of the buds was 15.9 cm,the fresh weight was 27.58 g,the chlorophyll content was 0.436 mg/g,the protein content was 8.9 g/kg,the soluble total sugar content was 23.86 g/kg,and the vitamin C content was 187.3 mg/kg.In production practice,considering the antibacterial activity and the growth of buds,the wood vinegar solution can be diluted 30 times and replaced with a culture medium for 10 days.

关 键 词：木醋 龙芽楤木 水培 抑菌作用 嫩芽生长 

分 类 号：S647[农业科学—蔬菜学]