糖络宁对高糖环境下雪旺细胞线粒体相关内质网膜的作用机制研究  

Study on effect of Tangluoning on Schwann cells mitochondria-associated membranes in high glucose environment

作　　者：朱晏伯 李潇 朱笳悦 刘浩龙 许利平[1,2] 杨鑫伟 ZHU Yan-bo;LI Xiao;ZHU Jia-yue;LIU Hao-long;XU Li-ping;YANG Xin-wei(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Beijing Key Lab of TCM Collateral Disease Theory Research,Beijing 100069,China;Pharmaceutical Department,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中医络病研究北京重点实验室,北京100069 [3]首都医科大学附属北京朝阳医院药事部,北京100020

出　　处：《中华中医药杂志》2021年第6期3187-3191,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81704014);北京市优秀人才青年骨干项目(No.2017000020124G29);国家自然科学基金面上项目(No.81873125);北京市自然科学基金项目(No.7194283)。

摘　　要：目的:观察糖络宁含药血清对高糖环境下雪旺细胞(SCs)线粒体相关内质网膜(MAMs)的影响。方法:采用高糖环境培养48h的SCs(RSC96细胞株)模型,设立空白对照组、模型对照组、α硫辛酸(ALA)组、1%糖络宁组、10%糖络宁组。在电镜观察MAMs形态的基础上,采用免疫荧光标记法定量检测SCs中MAMs。梯度超速离心法分离MAMs,通过ELISA法检测MAMs上PKR样内质网激酶(PERK)的表达、Western Blot检测MAMs上线粒体融合蛋白2(Mfn2)的表达。结果:与空白对照组比较,模型对照组MAMs数量降低、PERK表达显著升高、Mfn2表达显著降低(P<0.01);与模型对照组比较,1%、10%糖络宁组MAMs数量均显著增加,PERK表达降低而Mfn2表达显著升高(P<0.01)。结论:糖络宁能够增加高糖环境下SCs中MAMs,与其增加Mfn2,降低PERK的表达有关。Objective:To explore the effect of Tangluoning medicated serum in Schwann cells(SCs)mitochondriaassociated membranes(MAMs)incubated by high glucose.Methods:High glucose incubated SCs were used as cell models followed by setting control group,model group,Alpha lipoic acid(ALA)group,1%Tangluoning containing serum(LTLN)group and 10%Tangluoning containing serum(HTLN)group.Based on the observation of MAMs morphology by electron microscope,separation of MAMs,by gradient ultracentrifugation and detection of PKR-like endoplasmic reticulum kinase(PERK)expression on MAMs by ELISA.Western blot analysis was used to detect the expression of mitofusin 2(Mfn2)on MAMs in SCs.Results:Compared with control group,the number of MAMs decreased,the expression of PERK increased and the expression of Mfn2 decreased in model group(P<0.01).Compared with model group,the number of MAMs increased,the expression of PERK decreased and the expression of Mfn2 increased in LTLN group and HTLN group(P<0.01).Conclusion:Tangluoning can increase MAMs in SCs in high glucose environment,which is related to increasing Mfn2 and decreasing PERK expression.

关 键 词：糖络宁 糖尿病周围神经病变 雪旺细胞 线粒体相关内质网膜 线粒体融合蛋白2 PKR样内质网激酶 

分 类 号：R285[医药卫生—中药学]