祛风宣肺方对模型豚鼠肺组织病理及PLC-β/PKC与TRPA1/V1蛋白表达的影响  

Effects of Qufeng Xuanfei Decoction on the pathological changes and the expression of PLC-β/PKC,TRPA1/V1 proteins in lung tissue of model guinea pigs

作　　者：李佳珊 史利卿[2] 马建岭[2] 王丽云[2] 温绍惠[2] 董尚娟 季坤[2] 王颖[1] 宋欢[1] 杨凯[1] LI Jia-shan;SHI Li-qing;MA Jian-ling;WANG Li-yun;WEN Shao-hui;DONG Shang-juan;JI Kun;WANG Ying;SONG Huan;YANG Kai(Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China)

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《中华中医药杂志》2021年第6期3246-3250,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市自然科学基金项目(No.7184224,No.7192115);国家自然科学基金项目(No.81373588);北京中医药大学东方医院“1166”人才培养项目(No.040204001001002012);北京中医药大学科研基金项目(No.2017-JYB-JS-102,No.2018-JYBZZ-JS144)。

摘　　要：目的:观察祛风宣肺方对模型豚鼠肺组织病理及PLC-β/PKC、TRPA1/V1蛋白表达的影响。方法:将48只SPF级雄性健康Hartley豚鼠随机分为正常组、模型组、TRPV1抑制剂组、TRPA1抑制剂组、PLC抑制剂组、祛风宣肺方组,每组8只,应用经典咳嗽高敏感豚鼠模型,相应药物干预7d后,肺功能仪检测麻醉后豚鼠气道阻力,HE染色法观察肺组织病理变化,ELISA法检测BALF中SP水平,免疫组化法测定肺组织中PLC-β、PKC、TRPA1、TRPV1、NK-1R蛋白表达水平。结果:与模型组比较,各组气道阻力均显著降低(P<0.01,P<0.05),肺组织TRPA1、TRPV1、NK-1R蛋白表达显著降低(P<0.05,P<0.01),其中PLC抑制剂组与祛风宣肺方组肺组织PLC-β蛋白表达显著降低(P<0.01,P<0.05);模型组肺组织病理及BALF炎症反应明显,TRPV1抑制剂组、TRPA1抑制剂组、PLC抑制剂组及祛风宣肺方组炎症水平降低(P<0.01)。结论:祛风宣肺方可降低咳嗽敏感性增高模型豚鼠的气道反应性,减轻肺组织炎症,可能通过下调肺组织中PLC-β、TRPV1、TRPA1、NK-1R的蛋白表达水平发挥作用。Objective:To observe the effects of Qufeng Xuanfei Decoction on the pathological changes and the expression of PLC-β,PKC,TRPA1,TRPV1 proteins in lung tissue of model guinea pig.Methods:Forty-eight guinea pigs were randomly divided into blank control group,model group,TRPV1 inhibitor group,TRPA1 cnhibitor group,PLC inhibitor group,Qufeng Xuanfei group,8 in each group,the model of cough hyperactivity was established.After 7d-intervention,the airway reactivity was measured by spirometer under different concentrations of acetylcholine;the pulmonary pathology was observed by HE stain;the SP in BALF was detected by ELISA;the expression of PLC-β,PKC,TRPA1,TRPV1 proteins in lung tissue was detected by immunohistochemistry.Results:Compared with model group,the airway resistance and expression of lung TRPA1,TRPV1,NK-1R proteins in each group decreased significantly(P<0.01,P<0.05);PLC-βproteins in lung decreased significantly in PLC inhibitor group and Qufeng Xuanfei group(P<0.01,P<0.05).The lung tissue showed apparent structure destruction and airway inflammation in model group,while the TRPV1 inhibitor group,TRPA1 inhibitor group,PLC inhibitor group and Qufeng Xuanfei group showed a lower level of structure destruction and airway inflammation compared with model group(P<0.01).Conclusion:Qufeng Xuanfei Decoction could reduce the airway reactivity and inflammation of guinea pigs with cough hyperactivity,the therapeutic mechanism might be regulated by the expression of PLC-β,PKC,TRPA1,TRPV1 proteins in lung tissue.

关 键 词：祛风宣肺方 咳嗽高敏感 气道神经源性炎症 瞬时受体电位V1通道 瞬时受体电位A1通道 磷脂酶C 蛋白激酶C 

分 类 号：R285.5[医药卫生—中药学]