当归多糖对小鼠急性酒精性肝病的预防作用及对HIF-1α蛋白的影响  

Preventive effect of Angelica sinensis polysaccharide on acute alcoholic liver injury in mice and its influence on HIF-1αprotein

作　　者：曹鹏 张玉[1] 汪汉香 张琪琳[1] 李强[1] 王凯平[2] CAO Peng;ZHANG Yu;WANG Han-xiang;ZHANG Qi-lin;LI Qiang;WANG Kai-ping(Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;School of Pharmacy,Tongji Medical College of Huazhong University of Science&Technology,Wuhan 430000,China)

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022 [2]华中科技大学同济医学院药学院,武汉430000

出　　处：《中华中医药杂志》2021年第6期3628-3631,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81903901);国家重点研发计划项目(No.2017YFC0909900);国家自然科学基金面上项目(No.81974509)。

摘　　要：目的:观察当归多糖对小鼠急性酒精性肝病的预防作用及对HIF-1α蛋白的影响。方法:建立急性酒精性肝病C57BL/6小鼠模型,预防灌胃当归多糖(200mg/kg),处死小鼠后,眼眶采血分离血清,并收集肝脏组织。用化学比色法检测血清甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)水平和肝脏TG和谷胱甘肽(GSH)含量,进行肝脏HE、油红O、天狼星红和PAS染色切片观察肝脏病理学变化,Western Blot法和免疫组化法检测并观察肝脏HIF-1α的蛋白表达量。结果:当归多糖能够有效预防急性酒精性肝病,显著降低急性酒精性肝病小鼠血清ALT、AST和TG水平(P<0.05)。此外,小鼠肝脏HE和油红O染色切片显示,当归多糖能够完全预防肝细胞脂肪变性和脂肪堆积。进一步的机制研究表明,当归多糖的药理作用可能与其上调肝脏HIF-1α蛋白有关。结论:本研究首次发现了当归多糖预防酒精性肝病的药理作用,为酒精性肝病的药物防治策略提供了新的思路。This study had established a C57BL/6 mouse model of acute alcoholic liver disease,the pharmacological effects and molecular mechanism of Angelica sinensis polysaccharides(200mg/kg)were observed by oral administration.Serum and liver tissues were collected after the mice were sacrificed.The levels of serum triglyceride(TG),alanine aminotransferase(ALT)and aspartate transaminase(AST),along with hepatic TG and glutathione(GSH)were detected by chemical method.Liver pathological changes were observed by liver HE,oil red O and PAS staining slices.Western Blot and immunohistochemistry were used to detect and observe the protein expression of HIF-1α.The results revealed that ASP could effectively prevent liver damage and hyperlipidemia in model mice,significantly lower serum ALT,AST and TG levels(P<0.05).In addition,the liver HE,oil red O and PAS stained sections showed that ASP could completely prevent hepatic steatosis and fat accumulation.Further mechanistic studies revealed that the pharmacological efficacy of ASP might be related to the up-regulation of hepatic HIF-1αprotein expression.This study,for the first time,discovers the pharmacological effects of ASP in the prevention of alcoholic liver disease and provides new ideas for the drug treatment of alcoholic liver disease.

关 键 词：传统中药 活性成分 当归 多糖 酒精性肝病 药物防治 

分 类 号：R285.5[医药卫生—中药学]